Team:KULeuven/Data

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Revision as of 10:12, 21 September 2011

KULeuven iGEM 2011

Data page

How does E.D. Frosti work?

When Lactose is added to the medium, plasmid 1 will be induced to form INP via BBa_K584028.
When L-arabinose is added to the medium, plasmid 2 will be induced to form AFP via.

Data for our favorite new parts

1. BBa_K584028 (Main Page) - pLac-Lux hybrid promoter + INP , BBa_K584028 (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. We added 1mM IPTG to E.D. Frosti's medium. Water freezes immediately upon addition. Addition of a control (GFP construct) E.D. Frosti did not result in freezing. Note: The promoter coupled to GFP did not show any leakiness, but when we coupled the promoter to the INP we noticed a certain leakiness. The dual inhibition system is a way to get around this leakiness.

2. BBa_K584027 (Main Page) - Constitutive Promoter + INP , BBa_K584027 (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. Water freezes immediately upon addition. Addition of a control (GFP construct) E.D. Frosti did not result in freezing.

3. BBa_K584026 (Main Page) - constitutive promoter + AFP , BBa_K584026 (K.U.Leuven, iGEM 2011): We used the same conditions for the MilliQ water, but now at -10°C. Unfortunately addition of E.D. Frosti coated with AFP (or nothing: GFP control) did not result in prevention of ice formation. Due to lack of time we could not test other conditions (different temperatures, different concentrations,...)

Data for pre-existing parts

1. BBa_I13453 (Experience) - pBAD promoter, BBa_I13453 (Endy Lab, iGEM 2005): Fused a GFP reporter to this promoter (which created biobrick: BBa_K584000). The results can be found at the experience page of that promoter.

2. BBa_J23119 (Experience) - constitutive promoter, BBa_J23119 (Berkeley, iGEM 2006): Fused a GFP reporter to this promoter (which created biobrick: BBa_K584001). The results can be found at the experience page of that promoter.

We've also characterized the following parts

1. BBa_K584004 (Main Page) - HybB promoter + GFP generator, BBa_K584004 (KULeuven, iGEM 2011): We created this biobrick but due to lack of time we could not test it.

@@ Line 129: / Line 129: @@
 <h2>Data for our favorite new parts</h2>
-<li> 1. <b>BBa_K584028</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584028" target="blank"> (Main Page) </a> - <b> pLac-Lux hybrid promoter + INP </b>, <b> BBa_K584028 </b> (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. We added 1mM IPTG to <i>E.D. Frosti</i>'s medium. Water freezes immediately upon addition. Addition of a control (GFP construct) <i> E.D. Frosti </i> did not result in freezing.
+<li> 1. <b>BBa_K584028</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584028" target="blank"> (Main Page) </a> - <b> pLac-Lux hybrid promoter + INP </b>, <b> BBa_K584028 </b> (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. We added 1mM IPTG to <i>E.D. Frosti</i>'s medium. Water freezes immediately upon addition. Addition of a control (GFP construct) <i> E.D. Frosti </i> did not result in freezing. <u>Note</u>: The promoter coupled to GFP did not show any leakiness, but when we coupled the promoter to the INP we noticed a certain leakiness. The dual inhibition system is a way to get around this leakiness.
 <br><br>
 <li> 2. <b>BBa_K584027</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584027" target="blank"> (Main Page) </a> - <b> Constitutive Promoter + INP </b>, <b> BBa_K584027 </b> (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. Water freezes immediately upon addition. Addition of a control (GFP construct) <i> E.D. Frosti </i> did not result in freezing.