"As in Synbiome also in real life collaboration is the best way to success"
Cit: "The comunicator"
Edimburg IGEM team
During the theoretical work on Synbiome we decided to use a Beta Glucosidase as carbon source for our Interkingdom Consortium. We asked to Chris French to send us the BBa_K392008 a part made by Osaka 2010 encoding a β-glucosidase, from the bacterium Cellulomonas fimi. Firstly we tested the part and it works but during the generation of BBa_K553005 e BBa_K553006 we decided to sequence an intermediate version and the sequences underlined the presence of a frameshift at the beginning of the coding sequence, introducing a stop codon. We shared this information with Edimburg iGEM team and together we elaborated a solution to this strange problem, you can find more detailed information here at the Edimburg IGEM team cooperation page. They were also so kind to provide us a protocol for an assay that can be used to test for activity of this β-glucosidase, using 4-methylumbelliferyl-β-D-glucuronide.
Wageningen IGEM team
We also collaborated with the Wageningen iGEM team analyzing with Fluorescence Microscopy a bacterial strain expressing CYP . During the summer they typed on Twitter looking for a Confocal Microscope with laser and filter for CYP. Here at ICGEB, they have it and so we decided to contact the Wageningen IGEM team in order to help them analyzing this samples.This cooperation is also reported in the Cooperation page of Wageningen’s Wiki.
JUDGING
We believe our team deserves the Gold medal because we met the following criteria:
Requirements for a Bronze Medal: 1.Register the team, have a great summer, and plan to have fun at the Regional Jamboree. 2.Successfully complete and submit this iGEM 2011 Judging form. 3.Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts. 4.Plan to present a Poster and Talk at the iGEM Jamboree. 5.Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Standard Biological Parts. Including: 5.1.Primary nucleaic acid sequence 5.2.Description of function 5.3.Authorship 5.4.Safety notes, if relevant. 5.5.Acknowedgment of sources and references 6.Submit DNA for at least one new BioBrick Part or Device to the Registry.
Additional Requirements for a Silver Medal: 1.Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected; 2.Characterize the operation of your new part/device. 3.Enter this information and other documentation on the part's 'Main Page' section of the Registry. Part Number(s): BBa_K553000 BBa_K553001 BBa_K553002 BBa_K553005 BBa_K553007 BBa_K553008 BBa_K553009 BBa_K553020 BBa_K553021 BBa_K553022
Additional Requirements for a Gold Medal: 1.Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system. Click here for details. 2.Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation. Click here for details.
iGEM Prizes
Best Human Practice Advance
This has been the first presence of Trieste in iGEM and this meant quite a hard venture in building up a team, finding money, finding a hosting lab, designing and developing our project.
We students have made it all, obviously with the support of the University of Trieste but mostly with our passion and determination.Here in Trieste, for its history and location, we found an ideal place in which young students like us could undertake this innovative approach to research that iGEM represents.
We invested a great amount of energy on human practices because we aimed at creating the right environment to make iGEM a core activity among the first steps of the future researchers.
We decided to talk about iGEM and about our experiences as iGEM team through different channels and in different ways referring to a wide range of audience.
You can find a detailed description of UNITS_TRIESTE team human practices here!
IGEM more than a competition is a life experience; it involves you at all, sometimes it is hard sometimes funny but above all it is by far the best experience which a student could done during his undergraduate studies. The UNITS_TRIESTE Team is an “Handmade” team, no advisors working in synthetic biology field, no company linked to synthetic biology business only 6 students and their mighty wish to take their place in a worldwide acknowledged competition as IGEM is.
Synbiome is made of “undergraduate” thoughts and it has been realized by "undergraduate" hands.
As any researchers know three months are nothing, only 90 days tu build up a new, innovative and competitive project to face the world of synthetic biology.
We decided to do it as it must be do, no shortcuts: 6 month of preliminary work making reality the wish to partecipate at igem, learning about everything as biobricks, sponsors, registry, registration, human practice, tracks, jamboree, team and whatever else but mostly we thought about the project, tons of rough ideas falling down as bricks (Not yet so “Bio”J), looking for the best way to handle our first partecipation. At the end of june Synbiome was birth, son of all our minds and father of all our expectation.
Thanks to Synbiome we had the chance to involve scientists originally unrelated to the field of synthetic biology as our instructors. They believed so much our project that we’re not only working at Synbiome in a competitive optical but mostly looking forward to reach a publication at the end of the year.
These rows have been written only to give you all the feelings that this experience has meant for us, we really hope that you will enjoy Synbiome.
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