Team:UNITS Trieste/Acknowledgements
From 2011.igem.org
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<span>Edimburg IGEM team</span> | <span>Edimburg IGEM team</span> | ||
<div class="figure"><a href="https://2011.igem.org/Team:Edinburgh"><img style="padding-top:55px" width="200" src="https://static.igem.org/mediawiki/2011/d/d9/Edinburgh_home_logo.png"/></a></div> | <div class="figure"><a href="https://2011.igem.org/Team:Edinburgh"><img style="padding-top:55px" width="200" src="https://static.igem.org/mediawiki/2011/d/d9/Edinburgh_home_logo.png"/></a></div> | ||
- | <p>During the theoretical work on Synbiome we decided to use a Beta Glucosidase as carbon source for our Interkingdom Consortium. We asked to Chris French to send us the BBa_K392008 a | + | <p>During the theoretical work on Synbiome we decided to use a Beta Glucosidase as carbon source for our Interkingdom Consortium. We asked to Chris French to send us the BBa_K392008 a part made by Osaka 2010 encoding a β-glucosidase, from the bacterium Cellulomonas fimi. Firstly we tested the part and it works but during the generation of BBa_K553005 e BBa_K553006 we decided to sequence an intermediate version and the sequences underlined the presence of a frameshift at the beginning of the coding sequence, introducing a stop codon. We shared this information with Edimburg iGEM team and together we elaborated a solution to this strange problem, you can find more detailed information here at the Edimburg IGEM team cooperation page. They were also so kind to provide us a protocol for an assay that can be used to test for activity of this β-glucosidase, using 4-methylumbelliferyl-β-D-glucuronide.</p></div> |
<div class="cooperations" style="background-image:url(https://static.igem.org/mediawiki/2011/b/b2/Units-bkgR.png)" > | <div class="cooperations" style="background-image:url(https://static.igem.org/mediawiki/2011/b/b2/Units-bkgR.png)" > |
Revision as of 07:05, 21 September 2011