Team:Glasgow

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</script></div><p><br/>The DISColi project aims to design and construct a novel bio-photolithographic system for the engineering of biofilms into functional 2D and 3D structures and devices in response to different patterns and wavelengths of light.  <br/><br/>In this project we worked with light responsive promoters, a novel biofilm-forming synthetic biology chassis, <i>E. coli</i> Nissle 1917, and novel biobricks including several designed for biofilm dispersal and fluorescent reporters with wider utility than GFP. <br/><br/>The main aims of our project can be separated into three light-controlled components: the designed sculpting of biofilms; 3D printing for encapsulation of cells; and the controlled modular synthesis of a variety of products.<br/><br/> We expect this technology to have applications for material synthesis and compound manufacture in remote locations, for example outer space.
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<td><p>The DISColi project aims to design and construct a novel bio-photolithographic system for the engineering of biofilms into functional 2D and 3D structures and devices in response to different patterns and wavelengths of light </p><p>In this project we worked with light responsive promoters, a novel biofilm-forming synthetic biology chassis, <i>E. coli</i> Nissle 1917, and novel biobricks including several designed for biofilm dispersal and fluorescent reporters with wider utility than GFP.</p>
<td><p>The DISColi project aims to design and construct a novel bio-photolithographic system for the engineering of biofilms into functional 2D and 3D structures and devices in response to different patterns and wavelengths of light </p><p>In this project we worked with light responsive promoters, a novel biofilm-forming synthetic biology chassis, <i>E. coli</i> Nissle 1917, and novel biobricks including several designed for biofilm dispersal and fluorescent reporters with wider utility than GFP.</p>

Revision as of 17:01, 20 September 2011

DISColi: Bio-photolithography in Device Engineering Using Different Wavelengths of Light



RFP tagged E.coli Nissle biofilm

The DISColi project aims to design and construct a novel bio-photolithographic system for the engineering of biofilms into functional 2D and 3D structures and devices in response to different patterns and wavelengths of light

In this project we worked with light responsive promoters, a novel biofilm-forming synthetic biology chassis, E. coli Nissle 1917, and novel biobricks including several designed for biofilm dispersal and fluorescent reporters with wider utility than GFP.

 24-hour E.coli Nissle biofilm
Ranaspumin2 foam tagged with LOV

The main aims of our project can be separated into three light-controlled components: the designed sculpting of biofilms; 3D printing for encapsulation of cells; and the controlled modular synthesis of a variety of products.

We expect this technology to have applications for material synthesis and compound manufacture in remote locations, for example outer space.

 E.coli Nissle biofilm, with extracellular matrix




Sponsors

With many thanks to our generous sponsors, without whom this project would not have been possible.


Nexxus Sulsa Fisher Scientific Wellcome Trust

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