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Team:Groningen/project notebook/17 May 2011
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(Created page with "Joyce: <br> <br> Searching for protocols <br> Do a PCR for PhybB on E.coli DH5alpha suspension: <br> Composition: <br> Taq 10× buffer: 5μl <br> dNTPs: 1μl <br> MgCl2: 3μl <br...")
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Revision as of 09:30, 20 September 2011
Joyce:
Searching for protocols
Do a PCR for PhybB on E.coli DH5alpha suspension:
Composition:
Taq 10× buffer: 5μl
dNTPs: 1μl
MgCl2: 3μl
taq 5u/μl: 0.25μl
Forward primer 10mM: 1μl
Reverseprimer 10mM: 1μl
Template: 1μl
MQ: 38.75μl
PCR conditions:
denaturation: 10min
Cycle 25×
denaturtaion: 1min
annealing: 1min (gradient for 57 and 60 degrees)
extension: 1.5 min
Final extension: 10min
Store infinite
Analyse them on a 1% agarosegel
