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Team:Glasgow/iGEM Diary
From 2011.igem.org
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We had a meeting with <a href=http://www.gla.ac.uk:443/ibls/staff/staff.php?who=Ped|dQ>Dr Daniel Walker</a> who gave us some really useful information<br/> | We had a meeting with <a href=http://www.gla.ac.uk:443/ibls/staff/staff.php?who=Ped|dQ>Dr Daniel Walker</a> who gave us some really useful information<br/> | ||
We chose the biobricks we would need (various Promoters, Ribosome Binding Sites and Terminators)and began transforming them<br/> | We chose the biobricks we would need (various Promoters, Ribosome Binding Sites and Terminators)and began transforming them<br/> | ||
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<h1>Week 2 Summary</h1> | <h1>Week 2 Summary</h1> | ||
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PCR was carried out on our novel biobricks<br/> | PCR was carried out on our novel biobricks<br/> | ||
Our secret <i>E.coli</i> strain was successfully transformed with RFP! <br/><br/> | Our secret <i>E.coli</i> strain was successfully transformed with RFP! <br/><br/> | ||
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Revision as of 00:55, 20 September 2011
Want to know how life in the lab is going? Check out our weekly videos!
Week 1 Summary
This week the video is presented by Michal Przydacz. Click here to find out more about Michal!This week:
Our new lab was set up
We created glycerol stocks of E.coli and Pseudomonas strains for future use
We had a meeting with Dr Daniel Walker who gave us some really useful information
We chose the biobricks we would need (various Promoters, Ribosome Binding Sites and Terminators)and began transforming them
Week 2 Summary
This week the video is presented by Ellin Hillert. Click here to find out more about Ellin!This week:
We began the week with yet another transformation failure, resulting in our first successful transformants!
Our secret strain of E.coli arrived!
We each gave a presentation on different aspects of our project
Week 3 Summary
This week the video is presented by Andrew Landels. Click here to find out more about Andrew!This week:
We now have successful transformations of all our biobricks!
Our secret strain of E.coli forms really nice biofilms!
We had our very first Wiki Whaaaaaam!
Week 4 Summary
This week the video is presented by Pietro Ridone. Click here to find out more about Pietro!This week:
Work to gather data on biofilm growth and dispersal continues.
Primers for biobricks arrived
The guys have given us a sneak preview of the Norwich presentation
We have acquired our own Flouresence Microscope...
Week 5 Summary
This week the video is presented by Chris Wood. Click here to find out more about Chris!This week:
The guys have returned from Norwich with some great insights
Everyone is excited to incorporate human practices into the project in a new way
We now have a plan for Modelling
Week 6 Summary
This week the video is presented by Hannah Ralph. Click here to find out more about Hannah!This week:
We started ligating our constructs
PCR was carried out on our novel biobricks
Our secret E.coli strain was successfully transformed with RFP!
Week 7 Summary
This week the video is presented by Emma Campbell. Click here to find out more about Emma!This week:
Edinburgh sent us some biobricks to work with
We realised our novel biobricks have illegal restriction sites, so ordered primers for Site Directed Mutagenesis
Our poster for the Open Day is looking great! Amsterdam is booked - everyone is really excited!
Week 8 Summary
This week the video is presented by Stewart O'Neill. Click here to find out more about Stewart!This week:
Work on our light responsive constructs and Edinburgh biobricks is continuing
An EnvZ negative strain of E.coli arrived from the Registry
Modelling is going really well
Week 9 Summary
This week the video is presented by Scott Wood. Click here to find out more about Scott!This week:
PCR and Site Directed Mutagenesis is continuing on our novel biobricks
Our work with the EnvZ negative cells is ongoing
Modelling looks great - we now have some really great graphs for our dispersal biobricks!
