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Revision as of 21:28, 19 September 2011 (view source) Pjpatel (Talk | contribs) (Created page with "Safety: Initial Bleach Kill Curve") ← Older edit		Revision as of 21:48, 19 September 2011 (view source) Pjpatel (Talk | contribs) Newer edit →
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-	Safety:	+	'''Safety:'''
-	Initial Bleach Kill Curve	+
		+	''Initial Bleach Kill Curve''
		+
		+	Sample Bacteria for OD and initial cell number
		+	:-log phase culture  OD= .194
		+	:-stationary phase culture OD= .685
		+	:- No cell count for either (too many)
		+
		+	Experiment
		+
		+	-Mix 4.5 mL culture with 500λ bleach
		+
		+	-Incubate for 15 minutes and plate 1 mL
		+
		+	-Collect 1 mL and spin to pellet
		+
		+	-Wash twice with TE and resuspend in 100λ LB and then plate
		+
		+	:- Result: No Colonies
		+
		+
		+	Note: LB plates had bright pink colonies

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Revision as of 21:48, 19 September 2011

Safety:

Initial Bleach Kill Curve

Sample Bacteria for OD and initial cell number

-log phase culture OD= .194

-stationary phase culture OD= .685

- No cell count for either (too many)

Experiment

-Mix 4.5 mL culture with 500λ bleach

-Incubate for 15 minutes and plate 1 mL

-Collect 1 mL and spin to pellet

-Wash twice with TE and resuspend in 100λ LB and then plate

- Result: No Colonies

Note: LB plates had bright pink colonies