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Team:Greenfield IN-Schini-HS/May 25th, 2011
From 2011.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | Checked on primers | ||
| + | |||
| + | Did a laboratory check for procedures,materials, and safety protocols | ||
| + | |||
| + | Mapped out the next three weeks in the lab | ||
| + | |||
| + | 1. Isolation of DNA from yeast with primers for Gibson and primers for iGEM submission | ||
| + | 2. iGEM insertion for submission, using Gibson | ||
| + | 3. Gibson assembly into plasmid 416ura3 | ||
| + | 4. Transform into e.coli | ||
| + | 5. Transform into yeast | ||
| + | 6. Isolation of protein, Work on field process, Work on concentration controls | ||
| + | |||
Latest revision as of 20:35, 14 June 2011
Checked on primers
Did a laboratory check for procedures,materials, and safety protocols
Mapped out the next three weeks in the lab
1. Isolation of DNA from yeast with primers for Gibson and primers for iGEM submission 2. iGEM insertion for submission, using Gibson 3. Gibson assembly into plasmid 416ura3 4. Transform into e.coli 5. Transform into yeast 6. Isolation of protein, Work on field process, Work on concentration controls
