Team:UPO-Sevilla/Foundational Advances/MiniTn7/Bioinformatics/Whole phylogeny

From 2011.igem.org

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<p><strong>Figure 5</strong>. Glms protein multi-sequence alignment</p>
<p><strong>Figure 5</strong>. Glms protein multi-sequence alignment</p>
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<img class="center" width="400px" src="https://static.igem.org/mediawiki/2011/c/ca/UPOSevilla-BioInfor-Fig6.png" alt="Phylogenetic trees obtained for the figure 6 multi sequence alignment"/>
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<p><strong>Figure 6. A</strong>. Circular phylogenetic tree obtained for the figure 6 multi sequence alignment. <strong>B</strong>. Squared phylogenetic tree for the same sequences as in A.</p>
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<img class="center" width="400px" src="https://static.igem.org/mediawiki/2011/8/80/UPOSevilla-BioInfor-Fig7.jpg" alt="Bacteria, Archaea and Fungi logo"/>
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<p><strong>Figure 7. A</strong>. Bacteria and Archaea obtained logo for the last 16 amino acids of the C-terminal region of Glms. <strong>B</strong>. Fungi obtained logo for the last part of Glms.</p>
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Revision as of 17:00, 19 September 2011

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Results. Analysis throughout the whole phylogeny

Previously to the study of the codifying nucleotide glmS sequences, Glms protein was analyzed to check the reported high level of conservation of the C-terminal part of this protein (Milewski, 2002). Thus, a multialignment, a circular and squared tree and several logos of all the downloaded Glms sequences were obtained (figure 5). It is mentionable the high level of conservation found in the last 15 amino acids of this protein in all organisms which contrast with the lack of any conservation in the previous amino acidic sequence (this difference is perfectly shown in all sequence logos). It is also interesting that the consensus sequence of this C-terminal part of Glms protein match perfectly with the amino acidic sequence resulting when translating the nucleotide consensus obtained in figure 3.

Glms protein multi-sequence alignment

Figure 5. Glms protein multi-sequence alignment


Phylogenetic trees obtained for the figure 6 multi sequence alignment

Figure 6. A. Circular phylogenetic tree obtained for the figure 6 multi sequence alignment. B. Squared phylogenetic tree for the same sequences as in A.


Bacteria, Archaea and Fungi logo

Figure 7. A. Bacteria and Archaea obtained logo for the last 16 amino acids of the C-terminal region of Glms. B. Fungi obtained logo for the last part of Glms.