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Team:Wisconsin-Madison/biosensor
From 2011.igem.org
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Both the ethanol (EtOH) and n-alkane sensors rely on an arabinose inducible two plasmid system for either EtOH or n-alkane detection. The first plasmid contains genes for producing a protein controlled the pBAD promoter, which turns on transcription of the <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a> in the presence of arabinose. The second plasmid has the EtOH or n-alkane promoter which turns on the transcription of our RFP. The production of the RFP is then measured with a plate reader. | Both the ethanol (EtOH) and n-alkane sensors rely on an arabinose inducible two plasmid system for either EtOH or n-alkane detection. The first plasmid contains genes for producing a protein controlled the pBAD promoter, which turns on transcription of the <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a> in the presence of arabinose. The second plasmid has the EtOH or n-alkane promoter which turns on the transcription of our RFP. The production of the RFP is then measured with a plate reader. | ||
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In the <a href="https://2011.igem.org/Team:Wisconsin-Madison/directedevolution">directed evolution</a> construct, this two plasmid system is all put on a single plasmid, which the first plasmid’s parts being put on the reverse strand (5’ to 3’) of the DNA. | In the <a href="https://2011.igem.org/Team:Wisconsin-Madison/directedevolution">directed evolution</a> construct, this two plasmid system is all put on a single plasmid, which the first plasmid’s parts being put on the reverse strand (5’ to 3’) of the DNA. | ||
Revision as of 11:20, 19 September 2011
Project >>
Overview,
Ethanol Sensor,
Alkane Sensor,
Microcompartment
|
Biosensors
Both the ethanol (EtOH) and n-alkane sensors rely on an arabinose inducible two plasmid system for either EtOH or n-alkane detection. The first plasmid contains genes for producing a protein controlled the pBAD promoter, which turns on transcription of the genes in the presence of arabinose. The second plasmid has the EtOH or n-alkane promoter which turns on the transcription of our RFP. The production of the RFP is then measured with a plate reader.
In the directed evolution construct, this two plasmid system is all put on a single plasmid, which the first plasmid’s parts being put on the reverse strand (5’ to 3’) of the DNA. |
