Team:UNAM-Genomics Mexico/Parts

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m (→Parts: tested groupparts with iGEM2010)
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This is a template page. READ THESE INSTRUCTIONS.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<title>UNAM-Genomics_Mexico</title>
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:UNAM-Genomics_Mexico_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:UNAM-Genomics_Mexico | Team Example]]
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<!--- The Mission, Experiments --->
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!align="center"|[[Team:UNAM-Genomics_Mexico|Home]]
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!align="center"|[[Team:UNAM-Genomics_Mexico/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=UNAM-Genomics_Mexico Official Team Profile]
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!align="center"|[[Team:UNAM-Genomics_Mexico/Project|Project]]
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!align="center"|[[Team:UNAM-Genomics_Mexico/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:UNAM-Genomics_Mexico/Modeling|Modeling]]
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<li class="istylei0"><a class="istylei0" href="https://2011.igem.org/Team:UNAM-Genomics_Mexico">H2 Homepage</a></li>
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!align="center"|[[Team:UNAM-Genomics_Mexico/Notebook|Notebook]]
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<li class="istylei0"><a class="istylei0" href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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!align="center"|[[Team:UNAM-Genomics_Mexico/Safety|Safety]]
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<ul class="istylem0" style="width:195px;">
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!align="center"|[[Team:UNAM-Genomics_Mexico/Attributions|Attributions]]
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<li><a href="#"><span>Intro</span><![if gt IE 6]></a>
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<li><a href="#"><span>Theory</span><![if gt IE 6]></a>
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<li><a href="#"><span>Description</span><![if gt IE 6]></a>
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<li class="istylei0"><a class="istylei0" href="#">Abstract in a Picture</a></li>
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<li class="istylei0"><a class="istylei0" href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Team"><span>Team Members</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class="istylem0" style="width:195px;">
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<li><a href="https://igem.org/Team.cgi?year=2011&team_name=UNAM-Genomics_Mexico"><span>Oficial Team Profile</span><![if gt IE 6]></a>
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<li><a href="#"><span>Motivation</span><![if gt IE 6]></a>
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<li><a href="#"><span>Brainstorm</span><![if gt IE 6]></a>
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<li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Team"><span>Team Members</span><![if gt IE 6]></a>
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<ul class="istylem0" style="width:173px;">
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<li><a href="https://2011.igem.org/User:Jurquiza">Uriel</a></li>
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<li><a href="https://2011.igem.org/User:Nandomgu">Nando</a></li>
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<li><a href="#">Paz</a></li>
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<li><a href="https://2011.igem.org/User:Fibonacci_Lopez">Fabricio</a></li>
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<li><a href="https://2011.igem.org/User:Hmedina">Hector</a></li>
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<li><a href="https://2011.igem.org/User:Dgarcia">Daniela</a></li>
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<li><a href="https://2011.igem.org/User:Khronus">Daniel</a></li>
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<li><a href="https://2011.igem.org/User:Melissam">Melissita</a></li>
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<li><a href="https://2011.igem.org/User:Alecanda">Aaron</a></li>
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<li><a href="https://2011.igem.org/User:Atrevino">Abiel</a></li>
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<li><a href="https://2011.igem.org/User:Meli_arr">Meli</a></li>
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<li><a href="https://2011.igem.org/User:Paedugar">Pae</a></li>
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<li><a href="https://2011.igem.org/User:Vladimir">Vladimir</a></li>
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<li><a href="https://2011.igem.org/User:Gustavoruiz">Gustavo</a></li>
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<li><a href="https://2011.igem.org/User:Palatris">Pato</a></li>
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<li><a href="https://2011.igem.org/User:Hamarillo">Helena</a></li>
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<li><a href="https://2011.igem.org/User:Paxiveri">Paloma</a></li>
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<li><a href="https://2011.igem.org/User:Nalvarez">Nataly</a></li>
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<li><a href="https://2011.igem.org/User:Misticastillo">Misti</a></li>
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<li><a href="https://2011.igem.org/User:AraceliAM">Araceli</a></li>
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<li><a href="https://2011.igem.org/User:Ocarrillo">Omar</a></li>
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<li><a href="https://2011.igem.org/User:MarioV">Mario</a></li>
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<li class="istylei0"><a class="istylei0" href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Notebook"><span>Notebook</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class="istylem0" style="width:195px;">
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<li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Parts"><span>Parts</span><![if gt IE 6]></a>
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<li><a href="#"><span>Calendar</span><![if gt IE 6]></a>
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<li><a href="#"><span>Protocols</span><![if gt IE 6]></a>
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<li><a href="#"><span>Programs</span><![if gt IE 6]></a>
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<li><a href="#"><span>Wet-Lab</span><![if gt IE 6]></a>
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<li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Modeling"><span>Modeling</span><![if gt IE 6]></a>
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<li class="istylei0"><a class="istylei0" href="#"><span>Human Practices</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li><a href="#"><span>Ethics</span><![if gt IE 6]></a>
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<li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Safety"><span>Biosecurity-Biosafety</span><![if gt IE 6]></a>
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<li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Bio-SintetizArte"><span>BioArt</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class="istylem0" style="width:173px;">
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<li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Bio-SintetizArte">Call</a></li>
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<li><a href="#">Photos</a></li>
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<li><a href="#">Send a Phrase</a></li>
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</ul>
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</ul></li>
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<li class="istylei0"><a class="istylei0" href="#">Sponsors</a></li>
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</ul>
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===Parts===
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<div class="left_image">
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<img class="sub_project" alt="Our Parts Image" src="https://static.igem.org/mediawiki/2011/b/b9/Hydrogen_cell_500x500.jpg" title="Parts">
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New for iGEM 2010 is the ''groupparts'' tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
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<div class="summary_text">
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<p>The hydrogen production pathway is composed of four proteins.
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<groupparts>iGEM2010 UNAM-Genomics_Mexico</groupparts>
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Our <a class="parts" href="">hydrogenase</a> is the translational fusion of the gene codiffying a
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<a class="parts" href="">ferredoxin></a> and <a class="parts" href=""><i>hydA</i></a>.
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<a class="parts" href="">PFOR</a> is a Pyruvate Ferredoxin Oxido-Reductase that helps with the electron flux.
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The other two proteins are <a class="parts" href="">maturases</a>, i.e. enzymes required for the proper assembly of the  
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hydrogenase. These genes are organized in two operons. The first one comprises the maturase and PFOR and is regulated by
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the <a class="parts" href=""><i>Rizhobium etli</i></a> promoter <a class="parts" href="">pNifH1</a>.
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The second one is regulated by <a class="parts" href="">pLacZ</a> and contains the maturases.
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<a class="parts" href="">Periplasm export tags</a> were added to the hydrogenase and PFOR.</p>
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</div>
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<div class="descriptive_text">
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<h1 class="part_desc">Codon optimization</h1>
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<p>Our systems final host is <i>R. elti</i>. Given this, we <a class="parts" href="">optimized</a> our sequences for
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<i>R. etli</i>'s codon usage.</p>
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<h1 class="part_desc">Periplasm export tags</h1>
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<p>As a Gramm-negative bacterium, <i>Rhizobium etli</i> has a periplasmatic compartment. Proteins can be exported into it  
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with the appropriate <a class="parts" href="">peptide signal</a>.</p>
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<h1 class="part_desc">The Registry</h1>
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<p>Please visit the registry <a class="parts" href="http://partsregistry.org/Main_Page">page</a> if you want to consult
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our or other team parts speciffic properties.</p>
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<!--It would be nice and quick for users to consult our parts in the registry directly from links here-->
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Revision as of 01:29, 14 June 2011

UNAM-Genomics_Mexico

{{{content}}}

UNAM-Genomics_Mexico

Our Parts Image

The hydrogen production pathway is composed of four proteins. Our hydrogenase is the translational fusion of the gene codiffying a ferredoxin> and hydA. PFOR is a Pyruvate Ferredoxin Oxido-Reductase that helps with the electron flux. The other two proteins are maturases, i.e. enzymes required for the proper assembly of the hydrogenase. These genes are organized in two operons. The first one comprises the maturase and PFOR and is regulated by the Rizhobium etli promoter pNifH1. The second one is regulated by pLacZ and contains the maturases. Periplasm export tags were added to the hydrogenase and PFOR.

Codon optimization

Our systems final host is R. elti. Given this, we optimized our sequences for R. etli's codon usage.

Periplasm export tags

As a Gramm-negative bacterium, Rhizobium etli has a periplasmatic compartment. Proteins can be exported into it with the appropriate peptide signal.

The Registry

Please visit the registry page if you want to consult our or other team parts speciffic properties.

Retrieved from "http://2011.igem.org/Team:UNAM-Genomics_Mexico/Parts"