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Team:Kyoto/Notebook
From 2011.igem.org
(→Week4: Monday 22th - Sunday 28th August) |
(→Week3: Monday 15th - Sunday 21th August) |
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'''Thursday'''<br/> | '''Thursday'''<br/> | ||
| + | Nutritional Signal(Sugiura,Shimosaka & Okumura)<br/> | ||
| + | PCR Amplification of glnL and glnG from gDNA of E.coli<br/> | ||
| + | --Primers--<br/> | ||
| + | glnL<br/> | ||
| + | Left primer: tctagaggagactgctttatggcaac<br/> | ||
| + | Right primer: actagtaggaactatcgtcatcgactac<br/> | ||
| + | glnG<br/> | ||
| + | Left primer: tctagaggtgacgtttatgcaacga<br/> | ||
| + | Right primer: actagtacacacaagctgtgaatcactc<br/> | ||
'''Thursday'''<br/> | '''Thursday'''<br/> | ||
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<html><a name="lab-week4"></a></html> | <html><a name="lab-week4"></a></html> | ||
| + | |||
== Week4: Monday 22th - Sunday 28th August == | == Week4: Monday 22th - Sunday 28th August == | ||
'''Monday'''<br/> | '''Monday'''<br/> | ||
Revision as of 07:25, 19 September 2011
Lab Note
Week1: Monday 1st - Sunday 7th August
Monday
行った実験名：
使った試薬名、容量：
用いた機械：
行った人：
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Week2: Monday 8th - Sunday 14th August
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Week3: Monday 15th - Sunday 21th August
Monday
Tuesday
Wednesday
Thursday
Nutritional Signal(Sugiura,Shimosaka & Okumura)
PCR Amplification of glnL and glnG from gDNA of E.coli
--Primers--
glnL
Left primer: tctagaggagactgctttatggcaac
Right primer: actagtaggaactatcgtcatcgactac
glnG
Left primer: tctagaggtgacgtttatgcaacga
Right primer: actagtacacacaagctgtgaatcactc
Thursday
Friday
Saturday
Sunday
Week4: Monday 22th - Sunday 28th August
Monday
Tuesday
Wednesday
Thursday
Luminescence(Kusaba, Terada, Hara):ハエの走行性実験① ♂、紫外線×2回、緑×2回 ♀、紫外線×2回、緑×2回
Friday
Luminescence(Kusaba):ハエの走行性実験① ♂、赤外線×2回 ♀、赤外線×2回
Nutritional Signal(Hashiya):
Transformation of bellow parts.
1,4-17M:BBa_K325909(lux operon)
2,1-12M:BBa_E0240
3,2-17F:BBa_120260(low copy vector)
Saturday
Luminescence(Kusaba):ハエの走行性実験① ♂、赤×2回、青×2回 ♀、赤×2回、青×2回
Sunday
Week5: Monday 29th August - Sunday 4th September
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Week6: Monday 5th September - Sunday 11th September
Monday
Tuesday
Luminescence(Kusaba, Hara):ハエの走行性実験②（②は改良版） ♂、緑×2回 ♀、青×1回
Wednesday
Luminescence(Hara):ハエの走行性実験② ♂、青×2回 ♀、緑×2回、青×1回
Thursday
Luminescence(Hara):ハエの走行性実験② ♂、紫外線×3回 ♀、紫外線×3回
Friday
Saturday
Luminescence(Kusaba, Hara):ハエの走行性実験② ♂、青×2回、赤×2回 ♀、青×2回、赤×2回
Sunday
Luminescence(Kusaba, Hara):ハエの走行性実験② ♂、紫外線×2回、
Week7: Monday 12th September - Sunday 18th September
Monday
Luminescence:大腸菌の形質転換(Hashiya) ハエの走行性実験②(Kusaba, Hara)
Tuesday
Luminescence:大腸菌はじめて光る。しかし光量は少ない。
Wednesday
Thursday
Friday
Saturday
Sunday
Week8: Monday 19th September - Sunday 25th September
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Week9: Monday 26th September - Sunday 2nd October
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Protocol
Medium for drosophila
[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Medium for drosophila] Materials Methods - water : 500mL
- dry yeast : 20g
- corn flour : 45g
- glucose : 50g
- agarose : 3.5~5g
- propionic acid : 1.5mL
- 10% p-hydroxybenzoate in 70% Eternol : 5g
- Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it.
- Stir corn flour and glucose with the remaining water.
- Stir 1 and 2, then autoclave it again.
- after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.■
