Team:Kyoto/Notebook

From 2011.igem.org

(Difference between revisions)
(Protocol)
(Week4: Monday 22th - Sunday 28th August)
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'''Friday'''<br/>
'''Friday'''<br/>
Luminescence(Kusaba):ハエの走行性実験① ♂、赤外線×2回 ♀、赤外線×2回
Luminescence(Kusaba):ハエの走行性実験① ♂、赤外線×2回 ♀、赤外線×2回
 +
 +
Hungry(Hashiya):
 +
Transformation of bellow parts.
 +
1,4-17M:BBa_K325909(lux operon)
 +
2,1-12M:BBa_E0240
 +
3,2-17F:BBa_120260(low copy vector)
'''Saturday'''<br/>
'''Saturday'''<br/>

Revision as of 06:52, 19 September 2011

Contents

Lab Note

Week1: Monday 1st - Sunday 7th August

Monday
行った実験名:

使った試薬名、容量:

用いた機械:

行った人:


Tuesday

Wednesday

Thursday

Friday

Saturday

Sunday

Week2: Monday 8th - Sunday 14th August

Monday

Tuesday

Wednesday

Thursday

Friday

Saturday

Sunday

Week3: Monday 15th - Sunday 21th August

Monday

Tuesday

Wednesday

Thursday

Thursday

Friday

Saturday

Sunday

Week4: Monday 22th - Sunday 28th August

Monday

Tuesday

Wednesday

Thursday
Luminescence(Kusaba, Terada, Hara):ハエの走行性実験① ♂、紫外線×2回、緑×2回 ♀、紫外線×2回、緑×2回

Friday
Luminescence(Kusaba):ハエの走行性実験① ♂、赤外線×2回 ♀、赤外線×2回

Hungry(Hashiya): Transformation of bellow parts. 1,4-17M:BBa_K325909(lux operon) 2,1-12M:BBa_E0240 3,2-17F:BBa_120260(low copy vector)

Saturday
Luminescence(Kusaba):ハエの走行性実験① ♂、赤×2回、青×2回 ♀、赤×2回、青×2回

Sunday

Week5: Monday 29th August - Sunday 4th September

Monday

Tuesday

Wednesday

Thursday

Friday

Saturday

Sunday

Week6: Monday 5th September - Sunday 11th September

Monday

Tuesday
Luminescence(Kusaba, Hara):ハエの走行性実験②(②は改良版) ♂、緑×2回 ♀、青×1回

Wednesday
Luminescence(Hara):ハエの走行性実験② ♂、青×2回 ♀、緑×2回、青×1回

Thursday
Luminescence(Hara):ハエの走行性実験② ♂、紫外線×3回 ♀、紫外線×3回

Friday

Saturday
Luminescence(Kusaba, Hara):ハエの走行性実験② ♂、青×2回、赤×2回 ♀、青×2回、赤×2回 

Sunday
Luminescence(Kusaba, Hara):ハエの走行性実験② ♂、紫外線×2回、

Week7: Monday 12th September - Sunday 18th September

Monday

Luminescence:大腸菌の形質転換(Hashiya) ハエの走行性実験②(Kusaba, Hara)

Tuesday

Luminescence:大腸菌はじめて光る。しかし光量は少ない。

Wednesday

Thursday

Friday

Saturday

Sunday

Week8: Monday 19th September - Sunday 25th September

Monday

Tuesday

Wednesday

Thursday

Friday

Saturday

Sunday

Week9: Monday 26th September - Sunday 2nd October

Monday

Tuesday

Wednesday

Thursday

Friday

Saturday

Sunday

Protocol

Medium for drosophila

[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Medium for drosophila]
Materials Methods
  • water : 500mL
  • dry yeast : 20g
  • corn flour : 45g
  • glucose : 50g
  • agarose : 3.5~5g
  • propionic acid : 1.5mL
  • 10% p-hydroxybenzoate in 70% Eternol : 5g
  1. Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it.
  2. Stir corn flour and glucose with the remaining water.
  3. Stir 1 and 2, then autoclave it again.
  4. after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.■