Team:DTU-Denmark-2

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Revolutionizing Synthetic Biology

The DTU-Denmark 2 iGEM team has designed a novel standardized assembly system, called “Plug 'n' Play with DNA”, where any biological parts can be gathered without use of restriction enzymes and ligases. This will make synthetic biology faster and assembly of an expression vector possible within a few hours. We have created a library of standardized biological parts for mammalian cells and fungi ready to plug 'n' play.

Assembly Standard

Mammalian cells

Filamentous Fungi

Achievements

We have achieved to create a novel assembly standard system for BioBrick called Plug'n Play with DNA.

We submitted 49 new parts and 21 plasmids to the Registry of Standard Biological Parts.

We demonstrated the function of our system in the mammalian cell line U2-OS by fluorescence and confocal microscopy.

We demonstrated the function of our system in filamentous fungi Aspergilli by fluorescence and microscopy.

We demonstrated the function of our system in E. coli by helping the Copenhagen iGEM team.

We achieved to characterized two new fungal promoter by use of ß-galactosidase assays and Bradford assays.

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