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From 2011.igem.org

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Revision as of 14:40, 18 September 2011

PCR product purification

1. Mix 1 volume of sample with 2 volumes of NT buffer in an 1,5 ml Eppendorf tube.
2. Place a column into a 2 ml collection tube and load the sample,
3. Centrifuge at 11.000 g for 1 min.
4. Discard flow through and place the column back into the collection tube.
5. Add 600 µl NT3 buffer and centrifuge at 11.000 g for 1 min.
6. Discard flow through and place the column back into the collection tube.
7. Centrifuge at 11.000 g for 2 min to remove NT3 buffer. Discard flow through.
8. Place the column into a clean 1,5 ml Eppendorf tube.
9. Add 30 µl Elution Buffer NE and incubate at RT for 1 min to increase the yield of eluted DNA.
10. Centrifuge at 11.000 g for 1 min.