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Team:LMU-Munich/Lab Notebook
From 2011.igem.org
(Difference between revisions)
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=Primer= | =Primer= | ||
| - | <br><b>pnikA-E,N,X-fwd</b> GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC | + | {| |
| - | <br><b>pnikA-S-rev</b> CCGCTACTAGTAGACGATAAAAGACGCACAAGCC | + | |<br><b>pnikA-E,N,X-fwd</b> |
| - | <br><b>prcnA-E,N,X-fwd</b> GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca | + | |<br>GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC |
| - | <br><b>prcnA-S-rev</b> CCGCTACTAGTAcgcaccaagtaagatggcg | + | |- |
| - | <br><b>luxCDrev</b> CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT | + | |<br><b>pnikA-S-rev</b> |
| - | <br><b>PbrR-M,R-fwd</b> GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG | + | |<br>CCGCTACTAGTAGACGATAAAAGACGCACAAGCC |
| - | <br><b>PbrR-S,A-rev</b> AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG | + | |- |
| - | <br><b>pbrRT-S,A-rev</b> agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC | + | |<br><b>prcnA-E,N,X-fwd</b> |
| - | <br><b>pbRMut-fwd</b> tcgtgcgggattctccagggactgtcggactgc | + | |<br>GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca |
| - | <br><b>pbrMut-rev</b> tccgacagtccctggagaatcccgcacgattgggc | + | |- |
| - | <br><b>ppbrA-E,N,X-fwd</b> AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC | + | |<br><b>prcnA-S-rev</b> |
| - | <br><b>ppbrA-S-rev</b> GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC | + | |<br>CCGCTACTAGTAcgcaccaagtaagatggcg |
| - | <br><b>luxCDfor</b> GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA | + | |- |
| - | <br><b>luxBfor</b> ATGAAATTTGGATTGTATGAAATTTGGATTGT | + | |<br><b>luxCDrev</b> |
| - | <br><b>luxBrev</b> CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC | + | |<br>CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT |
| - | <br><b>luxEfor</b> GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA | + | |- |
| - | <br><b>luxErev</b> CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA | + | |<br><b>PbrR-M,R-fwd</b> |
| - | <br><b>iscSfor</b> AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC | + | |<br>GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG |
| - | <br><b>icsSrev</b> agtcactagtattaaccggtctattaatgatgagcccattcg | + | |- |
| - | <br><b>pabAfor</b> AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG | + | |<br><b>PbrR-S,A-rev</b> |
| - | <br><b>pabArev</b> agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC | + | |<br>AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG |
| - | <br><b>aurFfor</b> AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC | + | |- |
| - | <br><b>aurFrev</b> agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG | + | |<br><b>pbrRT-S,A-rev</b> |
| - | <br><b>ChrBAfor</b> AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC | + | |<br>agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC |
| - | <br><b>ChrBArev</b> agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG | + | |- |
| - | <br><b>melAfor</b> | + | |<br><b>pbRMut-fwd</b> |
| - | <br><b>melArev</b> | + | |<br>tcgtgcgggattctccagggactgtcggactgc |
| + | |- | ||
| + | |<br><b>pbrMut-rev</b> | ||
| + | |<br>tccgacagtccctggagaatcccgcacgattgggc | ||
| + | |- | ||
| + | |<br><b>ppbrA-E,N,X-fwd</b> | ||
| + | |<br>AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC | ||
| + | |- | ||
| + | |<br><b>ppbrA-S-rev</b> | ||
| + | |<br>GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC | ||
| + | |- | ||
| + | |<br><b>luxCDfor</b> | ||
| + | |<br>GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA | ||
| + | |- | ||
| + | |<br><b>luxBfor</b> | ||
| + | |<br>ATGAAATTTGGATTGTATGAAATTTGGATTGT | ||
| + | |- | ||
| + | |<br><b>luxBrev</b> | ||
| + | |<br>CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC | ||
| + | |- | ||
| + | |<br><b>luxEfor</b> | ||
| + | |<br>GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA | ||
| + | |- | ||
| + | |<br><b>luxErev</b> | ||
| + | |<br>CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA | ||
| + | |- | ||
| + | |<br><b>iscSfor</b> | ||
| + | |<br>AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC | ||
| + | |- | ||
| + | |<br><b>icsSrev</b> | ||
| + | |<br>agtcactagtattaaccggtctattaatgatgagcccattcg | ||
| + | |- | ||
| + | |<br><b>pabAfor</b> | ||
| + | |<br>AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG | ||
| + | |- | ||
| + | |<br><b>pabArev</b> | ||
| + | |<br>agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC | ||
| + | |- | ||
| + | |<br><b>aurFfor</b> | ||
| + | |<br>AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC | ||
| + | |- | ||
| + | |<br><b>aurFrev</b> | ||
| + | |<br>agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG | ||
| + | |- | ||
| + | |<br><b>ChrBAfor</b> | ||
| + | |<br>AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC | ||
| + | |- | ||
| + | |<br><b>ChrBArev</b> | ||
| + | |<br>agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG | ||
| + | |- | ||
| + | |<br><b>melAfor</b> | ||
| + | |<br>gatcTCTAGAtgGCCGGCGCGTGGCTGGTCGGCAAGCCG | ||
| + | |- | ||
| + | |<br><b>melArev</b> | ||
| + | |<br>gatcACCGGTGGCGGACACTATGGCTATTTCTAGC | ||
| + | |} | ||
Revision as of 14:16, 18 September 2011
Week 1
First Primers arrived. Doing lots of PCRs.
Week 2
Still doing PCRs and start restriction digests.
Week 3
First Biobrick is ready!
Week 4
Week 5
Week 6
Week 7
Week 8
Protocols
Primer
pnikA-E,N,X-fwd | GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC |
pnikA-S-rev | CCGCTACTAGTAGACGATAAAAGACGCACAAGCC |
prcnA-E,N,X-fwd | GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca |
prcnA-S-rev | CCGCTACTAGTAcgcaccaagtaagatggcg |
luxCDrev | CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT |
PbrR-M,R-fwd | GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG |
PbrR-S,A-rev | AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG |
pbrRT-S,A-rev | agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC |
pbRMut-fwd | tcgtgcgggattctccagggactgtcggactgc |
pbrMut-rev | tccgacagtccctggagaatcccgcacgattgggc |
ppbrA-E,N,X-fwd | AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC |
ppbrA-S-rev | GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC |
luxCDfor | GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA |
luxBfor | ATGAAATTTGGATTGTATGAAATTTGGATTGT |
luxBrev | CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC |
luxEfor | GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA |
luxErev | CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA |
iscSfor | AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC |
icsSrev | agtcactagtattaaccggtctattaatgatgagcccattcg |
pabAfor | AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG |
pabArev | agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC |
aurFfor | AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC |
aurFrev | agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG |
ChrBAfor | AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC |
ChrBArev | agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG |
melAfor | gatcTCTAGAtgGCCGGCGCGTGGCTGGTCGGCAAGCCG |
melArev | gatcACCGGTGGCGGACACTATGGCTATTTCTAGC |
