Team:Warsaw/Synthetic Cloning

From 2011.igem.org

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<h2>Project description</h2>
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<h2>Fast safe and efficient</h2>
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<div class="note">Synthetic Cloning</div>
<div class="note">Synthetic Cloning</div>
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<div>In order to fine-tune expression of genes used in our project we have conducted measurement of various ribosome binding sites included in 2010 spring distribution. Our list of measured parts includes RBSs both from Community and Anderson's collections. <br>We have used standard measurement kit composed of promoter BBa_J23100 and GFP+terminator part BBa_I130401. All measurements were carried out using flow cytometer. <br>Results are <a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">here</a></div>
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<div>Molecular cloning techniques require propagation of the construct in living cells that is transforming plasmids into e.g. E. coli and growing cultures over-night.  
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<li>It is a time-consuming process, </li>
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<li>genes toxic to E.coli can not be clones this way  </li>
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<li>and it results in creation of genetically modified organisms. </li>
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Revision as of 19:42, 17 September 2011

Example Tabs

Fast safe and efficient


Synthetic Cloning
Molecular cloning techniques require propagation of the construct in living cells that is transforming plasmids into e.g. E. coli and growing cultures over-night.
  • It is a time-consuming process,
  • genes toxic to E.coli can not be clones this way
  • and it results in creation of genetically modified organisms.

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