Team:NTNU Trondheim/LacI2

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==LacI2 (BBa_K292006)==
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We found and orderd this part from the parts registry.
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Since it had an RBS and double terminator it would save us from some cloning work.
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When testcutting the constructs containing BBa_K292006 we got some unexpected results.
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Therefore we decided to digest the brick with three differnt enzymes with restriction sites
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in the given biobrick-sequence in addition to EcoR1 and PstI in order to look at the biobrick length.
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Except from the sample cut with EcorR1 and PstI, the samples were also cut with BglI witch is a restriction site
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in the pSB1A2 backbone.
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Overwiev of restrctions and expected fragmentlengths:
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{|border="1"
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!Restriction enzymes
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!Expected lengths (bp)
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|-
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|EcoR1 + PstI
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|2053 (backbone) and 1303 (biobrick)
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|-
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|BglI + BclI
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|2032 + 1324
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|-
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|BglI + EcoRV
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|1760 + 1596
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|-
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|BglI + BanII
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|1835 + 1521
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|}

Revision as of 09:29, 17 September 2011



LacI2 (BBa_K292006)

We found and orderd this part from the parts registry. Since it had an RBS and double terminator it would save us from some cloning work.

When testcutting the constructs containing BBa_K292006 we got some unexpected results. Therefore we decided to digest the brick with three differnt enzymes with restriction sites in the given biobrick-sequence in addition to EcoR1 and PstI in order to look at the biobrick length.

Except from the sample cut with EcorR1 and PstI, the samples were also cut with BglI witch is a restriction site in the pSB1A2 backbone.

Overwiev of restrctions and expected fragmentlengths:

Restriction enzymes Expected lengths (bp)
EcoR1 + PstI 2053 (backbone) and 1303 (biobrick)
BglI + BclI 2032 + 1324
BglI + EcoRV 1760 + 1596
BglI + BanII 1835 + 1521












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