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Team:Michigan/Protocols
From 2011.igem.org
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[[Media: heat_shock.pdf | Heat shock transformation of standard competent cells.]] | [[Media: heat_shock.pdf | Heat shock transformation of standard competent cells.]] | ||
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'''Enzyme usage''' ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations. | '''Enzyme usage''' ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations. | ||
Revision as of 23:16, 10 June 2011
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Protocols
Basic protocols ::: These protocols are necessary for all lab operations.
Gels and electrophoresis ::: These protocols contain information on the production and usage of gels.
Transformation ::: These protocols contain information on the production, usage, and transformation of competent cells.
Production of electrocompetent cells
Electroporation of electrocompetent cells
Production of standard/QT competent cells
Quick Transformation (QT) of standard competent cells
Heat shock transformation of standard competent cells.
Enzyme usage ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.
