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Team:LMU-Munich/Project/Description
From 2011.igem.org
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| + | ==<font color="#9933CC">'''Overall project'''</font>== | ||
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Metals and especially heavy metals are highly prescribed in concentrations in the drinking water ordinance. Qualifying and quantifying these by standard chemical methods is costly and complicated. | Metals and especially heavy metals are highly prescribed in concentrations in the drinking water ordinance. Qualifying and quantifying these by standard chemical methods is costly and complicated. | ||
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Using these sensors from (mostly) bacteria we create biosensors by linking them to the expression of a reporter (e.g. green glowing by the green fluorescent protein GFP). To not only qualify but also to quantify the metals, it is also necessary to measure the output by given input (metal concentration) for each of these biosensors. Afterwards one can determine the metal concentration by measuring the output. | Using these sensors from (mostly) bacteria we create biosensors by linking them to the expression of a reporter (e.g. green glowing by the green fluorescent protein GFP). To not only qualify but also to quantify the metals, it is also necessary to measure the output by given input (metal concentration) for each of these biosensors. Afterwards one can determine the metal concentration by measuring the output. | ||
| - | The quantification needs heavy high-tech machinery ... something not always given ... especially in free field. So a qualification of metals with | + | The quantification needs heavy high-tech machinery ... something not always given ... especially in free field. So a qualification of metals with an easy-to-see output is also needed. |
In the end our team hopes to have not only a set of metallsensors for precise quantification of a group of (heavy) metalls, but also an outdoor kit for qualifying metalls in more remote areas. With these it might be more easy and cheaper to determine the content of metals in our drinking water. | In the end our team hopes to have not only a set of metallsensors for precise quantification of a group of (heavy) metalls, but also an outdoor kit for qualifying metalls in more remote areas. With these it might be more easy and cheaper to determine the content of metals in our drinking water. | ||
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| + | == Project Details== | ||
| + | '' | ||
| + | Einzelne Systeme erklären | ||
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| + | Bilder!!! | ||
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| + | Bild Wellplatte mit bunten Farben'' | ||
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| + | == The Experiments == | ||
| + | '' | ||
| + | working BioBrick erläutern'' | ||
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| + | == Results == | ||
| + | '' | ||
| + | welche BioBricks sind fertig und funktionieren hier reinschreiben!!!'' | ||
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Revision as of 22:43, 15 September 2011
Contents |
Overall project
Metals and especially heavy metals are highly prescribed in concentrations in the drinking water ordinance. Qualifying and quantifying these by standard chemical methods is costly and complicated.
Bacteria sense metals in their surrounding in order to change their expression profile or react in order to adapt and accomodate to their environment.
Using these sensors from (mostly) bacteria we create biosensors by linking them to the expression of a reporter (e.g. green glowing by the green fluorescent protein GFP). To not only qualify but also to quantify the metals, it is also necessary to measure the output by given input (metal concentration) for each of these biosensors. Afterwards one can determine the metal concentration by measuring the output.
The quantification needs heavy high-tech machinery ... something not always given ... especially in free field. So a qualification of metals with an easy-to-see output is also needed.
In the end our team hopes to have not only a set of metallsensors for precise quantification of a group of (heavy) metalls, but also an outdoor kit for qualifying metalls in more remote areas. With these it might be more easy and cheaper to determine the content of metals in our drinking water.
Project Details
Einzelne Systeme erklären
Bilder!!!
Bild Wellplatte mit bunten Farben
The Experiments
working BioBrick erläutern
Results
welche BioBricks sind fertig und funktionieren hier reinschreiben!!!
