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Team:Grinnell/Notebook
From 2011.igem.org
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===Week 2=== | ===Week 2=== | ||
Performed PCR on esp and rsaA genes and run on gel. Results showed successful amplification. | Performed PCR on esp and rsaA genes and run on gel. Results showed successful amplification. | ||
| + | |||
| + | Performed a transformation experiment to test the efficiency of our competent cells make in Week 1. Results were successful with about a 0.7 x 10^6 CFU/μg. | ||
| + | |||
| + | Cleaned up PCR product and digested esp, rsaA, and spB1C3 plasmid. Ligated esp, rsaA, and esp and rsaA to spB1C3 and transformed into ''E. coli'' Top10. | ||
Revision as of 22:16, 9 June 2011
Notebook
Week 1
PCR primers designed according to Biobrick specifications and ordered for rsaA C-terminal and WT esp. Because of the discrepancy in GC content between Staphylococcus epidermidis and Caulobacter, we also optimized the esp sequence for Caulobacter for later synthesis with a higher resulting GC content.
Prepared competent cells of E. coli Top10. (Protocol)
Streaked plates of Caulobacter, Staphylococcus aureus, and S. epidermidis.
Template DNA for PCR prepared from colonies using [http://www.bioventures.com/products.php?cid=10005| GeneReleaser].
Week 2
Performed PCR on esp and rsaA genes and run on gel. Results showed successful amplification.
Performed a transformation experiment to test the efficiency of our competent cells make in Week 1. Results were successful with about a 0.7 x 10^6 CFU/μg.
Cleaned up PCR product and digested esp, rsaA, and spB1C3 plasmid. Ligated esp, rsaA, and esp and rsaA to spB1C3 and transformed into E. coli Top10.
