Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme

Ozyme digestion

This protocol aims at cutting plasmids on specific restriction sites using restriction enzymes.

Procedure

1. In an eppendorf tube, add the following solutions in any order :
-11µL water
-10µL DNA
-2.5µL NE Buffer 2
-0.5µL BSA X50

2. Add 0.5µL of each one of the desired enzymes.

3. Incubate at 37°C for 10mn

4. Inactivate the enzymes by a 20mn incubation at 80°C