"
Team:WarrenCIndpls IN-HS/Notebook
From 2011.igem.org
(→May 26th: Primer Design) |
(→May 19th: Research on Parts) |
||
| Line 34: | Line 34: | ||
==May 19th: Research on Parts== | ==May 19th: Research on Parts== | ||
| - | Kozak Sequence- directs translation of mRNA for more efficiency and accuracy | + | Kozak Sequence- directs translation of mRNA for more efficiency and accuracy; the amount of protein synthesized from mRNA is dependent on the strength of the Kozak sequence |
Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors | Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors | ||
Revision as of 13:20, 9 June 2011
| You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. |  |
|
Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs) | File:WarrenCIndpls IN-HS team.png Your team picture |
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Notebook
May 4th: Bacterial and Yeast Transformation
May 11th: Gibson Assembly
May 19th: Research on Parts
Kozak Sequence- directs translation of mRNA for more efficiency and accuracy; the amount of protein synthesized from mRNA is dependent on the strength of the Kozak sequence
Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors
Terminator Sequence- signals the end of transcriptoin to RNA polymerase
Vector- Plasmid
ORI (Origin of Replication)
Selection Markers
-Ura3 is a selection marker for yeast
-Ampicillin Resistance is a selection marker for bacteria based antibiotic resistance
May 26th: Primer Design
1st Forward Primer contains an overhang to attach the biobrick to the plasmid, two restriction enzyme sites (required to form a biobrick), and a primer for the constructin of a new strand
2nd Forward Primer contains a primer for contructing a new strand to connect the translational unit to the biobrick
Reverse Primer contains an overhang to attach the biobrick to the multi-cloning site (on the plasmid), the other two restriction enzymes sites, and a primer for contructiong a new strand
