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Team:Paris Liliane Bettencourt/Notebook/2011/09/13/
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*S105 in S27 x2 (one of the MPs didn't show satisfactory sequencing results) | *S105 in S27 x2 (one of the MPs didn't show satisfactory sequencing results) | ||
*S27 (-)ctrl for the two tubes that I used. (one concentrated at 9.8, the other at 5.4) | *S27 (-)ctrl for the two tubes that I used. (one concentrated at 9.8, the other at 5.4) | ||
| + | |||
| + | == Cyrille and Antoine == | ||
| + | |||
| + | Preparation of the last step of the ligation. | ||
| + | |||
| + | 12 miniprep of pHM3 were done and concentration measured. | ||
| + | 2 times 500 ng of each plasmid was digested in EP and runned on a gel. The bands where unclear. Only one miniprep seems to hold the correct insert altough there is a blur band for each of them at thee good size.this band was extracted and DNA was purified. | ||
| + | |||
| + | Treatment of the sequences - kinA project abandonned due to a wrongregsitry part | ||
| + | |||
| + | Digestion of PVeg ComS GA/PCR in EX, all the trnas (the digestion failed once), RBS frp ter, pT7 GFP in EX and SP (failed), S24 CI | ||
Revision as of 19:57, 13 September 2011
Contents |
Danyel
S38 in S24
Transformation failed. Positive control and negative control were good but no colonies for neither 20µL nor 200µL.
Sequencing results
- S98 : clone 1 and 6
- S99 : clone 2
- S92 : the results are correct
Ligation & Transformation
- S79 in S27
- S105 in S27 x2 (one of the MPs didn't show satisfactory sequencing results)
- S27 (-)ctrl for the two tubes that I used. (one concentrated at 9.8, the other at 5.4)
Cyrille and Antoine
Preparation of the last step of the ligation.
12 miniprep of pHM3 were done and concentration measured. 2 times 500 ng of each plasmid was digested in EP and runned on a gel. The bands where unclear. Only one miniprep seems to hold the correct insert altough there is a blur band for each of them at thee good size.this band was extracted and DNA was purified.
Treatment of the sequences - kinA project abandonned due to a wrongregsitry part
Digestion of PVeg ComS GA/PCR in EX, all the trnas (the digestion failed once), RBS frp ter, pT7 GFP in EX and SP (failed), S24 CI
