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Team:Caltech/Notebook
From 2011.igem.org
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<p>[[Team:Caltech/Week_11|Week 11]]: 8/21-8/27: Assembly of DDT-degradation gene and p450 into BioBrick submission and pET protein expression vectors</p> | <p>[[Team:Caltech/Week_11|Week 11]]: 8/21-8/27: Assembly of DDT-degradation gene and p450 into BioBrick submission and pET protein expression vectors</p> | ||
<p>[[Team:Caltech/Week_12|Week 12]]: 8/28-9/3: Biofilm-X-gal experiments, sequencing, HPLC</p> | <p>[[Team:Caltech/Week_12|Week 12]]: 8/28-9/3: Biofilm-X-gal experiments, sequencing, HPLC</p> | ||
| - | <p>[[Team:Caltech/Week_13|Week 13]]: 9/4-9/10</p> | + | <p>[[Team:Caltech/Week_13|Week 13]]: 9/4-9/10: Quikchange, Biobrick, sequencing</p> |
| + | <p>[[Team:Caltech/Week_14|Week 14]]: 9/12-9/18: Redo Quikchange, Biofilm, Protein Expression, HPLC</p> | ||
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Revision as of 18:42, 13 September 2011
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NotebookWeek 1: 6/15-6/17: Lab orientation Week 2: 6/19-6/25: Lab training; sample collection, enrichment cultures, DNA extraction from samples Week 3: 6/26-7/2: Prepare parts by PCR for Gibson assembly of test plasmids of BisdA and BisdB, continue enrichment cultures Week 4: 7/3-7/10: Sequencing and testing of Gibson assembled vectors Week 5: 7/11-7/16: Remake Gibson vectors, begin 16S sequencing, run pulsed field gel to extract degradation genes for fosmid kit Week 6: 7/17-7/23: Continuation of Gibson assembly, 16s sequencing and assembly, and fosmid kit Week 7: 7/24-7/30: p450 testing, plating of EDC's, more assembly Week 8: 7/31-8/6: Titering of fosmid kits, HPLC of p450 degradation, more assembly and EDC plating Week 9: 8/7-8/13 GCMS attempts at p450-degraded BPA, biofilm/crystal violet experiments, assembly of constructs and DDT gene Week 10: 8/14-8/20: Electrospray of p450-degraded BPA; visits to local water treatment plants; assembly of constructs, lacZ, and DDT gene Week 11: 8/21-8/27: Assembly of DDT-degradation gene and p450 into BioBrick submission and pET protein expression vectors Week 12: 8/28-9/3: Biofilm-X-gal experiments, sequencing, HPLC Week 13: 9/4-9/10: Quikchange, Biobrick, sequencing Week 14: 9/12-9/18: Redo Quikchange, Biofilm, Protein Expression, HPLC
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