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Team:Grinnell/Notebook
From 2011.igem.org
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===Week 1=== | ===Week 1=== | ||
| - | PCR primers designed according to Biobrick specifications and ordered for rsaA C-terminal and WT esp. Because of the discrepancy in GC content between ''Staphylococcus epidermidis'' and ''Caulobacter'', we also optimized the sequence | + | PCR primers designed according to Biobrick specifications and ordered for ''rsaA'' C-terminal and WT ''esp''. Because of the discrepancy in GC content between ''Staphylococcus epidermidis'' and ''Caulobacter'', we also optimized the ''esp'' sequence for ''Caulobacter'' for later synthesis with a higher resulting GC content. |
| - | Prepared competent cells of ''E. coli'' Top10. | + | Prepared competent cells of ''E. coli'' Top10. ([[Team:Grinnell/Notebook/Protocols#Competent|Protocol]]) |
| - | + | Streaked plates of ''Caulobacter'', ''Staphylococcus aureus'', and ''S. epidermidis''. | |
Template DNA for PCR prepared from colonies using [http://www.bioventures.com/products.php?cid=10005| GeneReleaser]. | Template DNA for PCR prepared from colonies using [http://www.bioventures.com/products.php?cid=10005| GeneReleaser]. | ||
===Week 2=== | ===Week 2=== | ||
Revision as of 23:53, 8 June 2011
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Notebook
Week 1
PCR primers designed according to Biobrick specifications and ordered for rsaA C-terminal and WT esp. Because of the discrepancy in GC content between Staphylococcus epidermidis and Caulobacter, we also optimized the esp sequence for Caulobacter for later synthesis with a higher resulting GC content.
Prepared competent cells of E. coli Top10. (Protocol)
Streaked plates of Caulobacter, Staphylococcus aureus, and S. epidermidis.
Template DNA for PCR prepared from colonies using [http://www.bioventures.com/products.php?cid=10005| GeneReleaser].
