"
Team:Grinnell/Notebook
From 2011.igem.org
(Difference between revisions)
| Line 15: | Line 15: | ||
<!--- The Mission, Experiments ---> | <!--- The Mission, Experiments ---> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
==Notebook== | ==Notebook== | ||
| - | + | ===Week 1=== | |
| + | PCR primers designed according to Biobrick specifications and ordered for rsaA C-terminal and WT esp. Because of the discrepancy in GC content between ''Staphylococcus epidermidis'' and ''Caulobacter'', we also optimized the sequence for esp for ''Caulobacter'' codons for later synthesis. | ||
| - | + | Prepared competent cells of ''E. coli'' Top10. | |
| - | [ | + | Prepared plates of ''Caulobacter'', ''Staphylococcus aureus'', and ''S. epidermidis'' using three-streak method. |
| + | |||
| + | Template DNA for PCR prepared from colonies using [http://www.bioventures.com/products.php?cid=10005| GeneReleaser]. | ||
| + | |||
| + | ===Week 2=== | ||
Revision as of 21:13, 8 June 2011
| You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. |  |
|
Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs) | File:Grinnell team.png Your team picture |
| Team Example |
Notebook
Week 1
PCR primers designed according to Biobrick specifications and ordered for rsaA C-terminal and WT esp. Because of the discrepancy in GC content between Staphylococcus epidermidis and Caulobacter, we also optimized the sequence for esp for Caulobacter codons for later synthesis.
Prepared competent cells of E. coli Top10.
Prepared plates of Caulobacter, Staphylococcus aureus, and S. epidermidis using three-streak method.
Template DNA for PCR prepared from colonies using [http://www.bioventures.com/products.php?cid=10005| GeneReleaser].
