"
Team:EPF-Lausanne/Todo
From 2011.igem.org
(Difference between revisions)
(→Wiki) |
(→= General) |
||
| Line 28: | Line 28: | ||
* Describe cell cultures in [[Team:EPF-Lausanne/Protocols/Miniprep|miniprep protocol]] | * Describe cell cultures in [[Team:EPF-Lausanne/Protocols/Miniprep|miniprep protocol]] | ||
| - | + | === General === | |
* Write a new protocol! | * Write a new protocol! | ||
* Write-up team presentation | * Write-up team presentation | ||
Revision as of 20:01, 8 June 2011
Todo
Contents |
Microfluidic Chips
- Continue alignment training
Preparing the parts
- Investigate failure of lysis device sequencing.
Next steps:
-
Make competent cells -
Test competent cells~10^5CFU/µg not very good -
Transform in E. Coli. - Glycerol Stocks
-
Plasmid preps - Sequence the lysis cassette
Assembly
-
Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP? - Design Gibson primers to assemble the three different plasmids.
- Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid".
Wiki
Protocols
For somebody who's made some cell cultures, please provide a sentence or two on how they're done, in the Miniprep protocol:
- Describe cell cultures in miniprep protocol
General
- Write a new protocol!
- Write-up team presentation
Clean rooms
- Order lab notebook
- Order storage box
