Meeting
attendants: Jakob, Julia, Manuel, Rüdiger, Sandra, Sophie, Theo
Time: 9:00 - 10:00
green light receptor
already done:
- Ccas some colonies (check if positive)
- CcaR still doesn't work
To-do:
- cph8 still won't work, we should ask to get the original sequence
blue light receptor
already done:
- receptor and NOT-Gate assembly
To-do:
- add Promotor-RBS and terminator
red light receptor
already done:
- Promotor-RBS-ho1-terminator ready
- Promotor-RBS-pcyA-terminator ready
To-do:
- receptor still doesn't work (not possible to amplify via a PCR)
Lysis cassette
already done:
- quick change with the 11 bases didn't work
- did a 3A assembly with the single parts
To-do:
Precipitator
already done:
- finally the genes arrived
- GST from the bioss toolkit can't be amplified from the supported vector, Rüdiger should do a positive control next time, if it still doesn't work he should get new primer pairs
To-do:
other stuff
- Hauke Busch recommended CellDesigner to Rüdiger to do the modelling, Rüdiger will download it and try to install it
- modelling should be done for the precipitator, escpecially the Ni-Histidin-binding and for the plastic binding domain, maybe also the GST-Tag affinity
- Tobi will organize moving the stuff out of the lab after the wiki freeze
- Sandra will ask for the appointment to talk at the MPI
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
Sandra, Sophie: primer design for NOT-gate to get NEH I restriction sites inside instead of Spe I. We can not cut with Spe because our trp-promoter contains Spe restriction sites.
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
3A-assembly
1.Digestion
Investigators: Rüdiger
| Sample name
| DNA concentration (ng/μl)
|
| M61a
| 335
|
| M61b
| 300
|
| M62a
| 267
|
| M62b
| 263
|
| M63a
| 447
|
| M63b
| 412
|
diegsted with EcoRI and PstI.
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