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Team:UC Davis/Notebook/Week 12
From 2011.igem.org
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The first characterizing run will be for our "good mutants" #1-6 at 0% arabinose. The four levels of IPTG that we have decided to start with are 0 mM, 0.25 mM, 0.5 mM, and 1 mM. | The first characterizing run will be for our "good mutants" #1-6 at 0% arabinose. The four levels of IPTG that we have decided to start with are 0 mM, 0.25 mM, 0.5 mM, and 1 mM. | ||
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| + | --Thursday 9/01/11-- | ||
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| + | We are starting to gather data at a very fast pace. The current schedule is to let each plate incubate for either 6 hours at 37 °C or 18 hours overnight at room temperature, and then to run each plate for 6 hours in the Tecan at 37 °C. This allows us to get in three runs a day: one at 8:00 am, one at 2:00 pm, and one at 8:00 pm. Thankfully we have a hardworking team that is dedicated to keeping the Tecan running 24/7! | ||
Revision as of 23:16, 12 September 2011
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View our judging criteria for iGEM 2011 here.--Monday 8/29/11--
--Wednesday 8/31/11--
Today marked the beginning of our characterization of R0010 mutants. We plan on characterizing six mutants at a time by running many 96-well plates (8 rows, 12 columns) through the Tecan. We will have six rows for mutants, one row for wild type, and a final row for our controls. Each plate will have a constant arabinose level in all wells (except the controls) and will have 4 levels of IPTG induction across each row. Not only will we be able to get a lot of data about how each mutant responds to various repressor concentrations, but we will determine a good range of arabinose and IPTG levels to test over in the upcoming weeks.
The first characterizing run will be for our "good mutants" #1-6 at 0% arabinose. The four levels of IPTG that we have decided to start with are 0 mM, 0.25 mM, 0.5 mM, and 1 mM.
--Thursday 9/01/11--
We are starting to gather data at a very fast pace. The current schedule is to let each plate incubate for either 6 hours at 37 °C or 18 hours overnight at room temperature, and then to run each plate for 6 hours in the Tecan at 37 °C. This allows us to get in three runs a day: one at 8:00 am, one at 2:00 pm, and one at 8:00 pm. Thankfully we have a hardworking team that is dedicated to keeping the Tecan running 24/7!
