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Sensor: Week 2 May 23-27
From 2011.igem.org
(Difference between revisions)
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| + | ==Thursday== | ||
| + | {|border="1" | ||
| + | !Assembly Step | ||
| + | |colspan="2" align="center"|Part Involved with Assembly Step | ||
| + | |- | ||
| + | |Miniprep for<br />Sequencing | ||
| + | |style="color:green" colspan="2" align="center"|B0034+Mcherry+B0015<br />J23113+B0034+C0051<br />J23105+B0034+C0051 | ||
| + | |- | ||
| + | |PCR | ||
| + | |colspan="2" align="center"|B0034+Mcherry+B0015 | ||
| + | |- | ||
| + | |rowspan="2"|Restriction Digest | ||
| + | |Insert using EcoRI and SpeI: | ||
| + | |B0034+Mcherry+B0015 | ||
| + | |- | ||
| + | |Vectors using EcoRI and XbaI: | ||
| + | |J23113+B0034+C0051<br />J23105+B0034+C0051 | ||
| + | |- | ||
| + | |Ligation | ||
| + | |colspan="2" | B0034+Mcherry+B0015 and J23113+B0034+C0051<br />B0034+Mcherry+B0015 and J23105+B0034+C0051 | ||
| + | |- | ||
| + | |Transformation/Plating | ||
| + | |colspan="2" | The above ligations were transformed into Escherichia<br />coli cells and plated onto Amp resistant plates. | ||
| + | |} | ||
Revision as of 22:51, 7 June 2011
Contents |
Monday
| Assembly Step | Parts Involved in Assembly Step |
|---|---|
| PCR | J23113 J23105 J23118 J23100 |
Tuesday
| Assembly Step | Parts Involved in Assembly Step | |
| PCR Purification | J23113 J23105 J23118 J23100 | |
| Miniprep | B0051 | |
| Restriction Digest | Inserts using EcoRI and SpeI: | J23113 J23105 J23118 J23100 B0034+Mcherry |
| Vectors using EcoRI and XbaI: | B0034+C0051 B0015 | |
| Ligation | J23113 and B0034+C0051 J23105 and B0034+C0051 J23118 and B0034+C0051 J23100 and B0034+C0051 B0034+Mcherry and B0015 | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto Amp resistant plates. | |
| PCR | B0030 B0031 B0032 B0033 | |
Wednesday
| Assembly Step | Parts Involved with Assembly Step | |
|---|---|---|
| Colony PCR | B0034+Mcherry+B0015 (Kan resistance) B0034+Mcherry+B0015 (Amp resistance) | |
| PCR Purification | B0030 B0031 B0032 B0033 | |
| Restriction Digest | Inserts using EcoRI and SpeI: | B0030 B0031 B0032 B0033 |
| Vector using EcoRI and XbaI: | C0051 | |
| Ligation | B0030+C0051 B0031+C0051 B0032+C0051 B0033+C0051 | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto Amp resistant plates. | |
Thursday
| Assembly Step | Part Involved with Assembly Step | |
|---|---|---|
| Miniprep for Sequencing | B0034+Mcherry+B0015 J23113+B0034+C0051 J23105+B0034+C0051 | |
| PCR | B0034+Mcherry+B0015 | |
| Restriction Digest | Insert using EcoRI and SpeI: | B0034+Mcherry+B0015 |
| Vectors using EcoRI and XbaI: | J23113+B0034+C0051 J23105+B0034+C0051 | |
| Ligation | B0034+Mcherry+B0015 and J23113+B0034+C0051 B0034+Mcherry+B0015 and J23105+B0034+C0051 | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto Amp resistant plates. | |
