Sensor: Week 2 May 23-27
From 2011.igem.org
(Difference between revisions)
(→Tuesday) |
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|Transformation/Plating | |Transformation/Plating | ||
- | |colspan="2" | The above | + | |colspan="2" | The above ligations were transformed into Escherichia<br />coli cells and plated onto Amp resistant plates. |
|- | |- | ||
|PCR | |PCR | ||
|colspan="2" align="center"| B0030<br />B0031<br />B0032<br />B0033 | |colspan="2" align="center"| B0030<br />B0031<br />B0032<br />B0033 | ||
+ | |} | ||
+ | |||
+ | ==Wednesday== | ||
+ | {|border="1" | ||
+ | !Assembly Step | ||
+ | |colspan="2" align="center"| '''Parts Involved with Assembly Step''' | ||
+ | |- | ||
+ | |Colony PCR | ||
+ | |colspan="2" | B0034+Mcherry+B0015 (Kan resistance)<br />B0034+Mcherry+B0015 (Amp resistance) | ||
+ | |- | ||
+ | |PCR Purification | ||
+ | |colspan="2" align="center"| B0030<br />B0031<br />B0032<br />B0033 | ||
+ | |- | ||
+ | |rowspan="2" | Restriction Digest | ||
+ | |Inserts using EcoRI and SpeI: | ||
+ | |B0030<br />B0031<br />B0032<br />B0033 | ||
+ | |- | ||
+ | |Vector using EcoRI and XbaI: | ||
+ | |C0051 | ||
+ | |- | ||
+ | |Ligation | ||
+ | |colspan="2" align="center"| B0030+C0051<br />B0031+C0051<br />B0032+C0051<br />B0033+C0051 | ||
+ | |- | ||
+ | |Transformation/Plating | ||
+ | |colspan="2" | The above ligations were transformed<br />into Escherichia coli cells and plated onto<br />Amp resistant plates. | ||
|} | |} | ||
Revision as of 22:42, 7 June 2011
Monday
Assembly Step | Parts Involved in Assembly Step |
---|---|
PCR | J23113 J23105 J23118 J23100 |
Tuesday
Assembly Step | Parts Involved in Assembly Step | |
PCR Purification | J23113 J23105 J23118 J23100 | |
Miniprep | B0051 | |
Restriction Digest | Inserts using EcoRI and SpeI: | J23113 J23105 J23118 J23100 B0034+Mcherry |
Vectors using EcoRI and XbaI: | B0034+C0051 B0015 | |
Ligation | J23113 and B0034+C0051 J23105 and B0034+C0051 J23118 and B0034+C0051 J23100 and B0034+C0051 B0034+Mcherry and B0015 | |
Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto Amp resistant plates. | |
PCR | B0030 B0031 B0032 B0033 |
Wednesday
Assembly Step | Parts Involved with Assembly Step | |
---|---|---|
Colony PCR | B0034+Mcherry+B0015 (Kan resistance) B0034+Mcherry+B0015 (Amp resistance) | |
PCR Purification | B0030 B0031 B0032 B0033 | |
Restriction Digest | Inserts using EcoRI and SpeI: | B0030 B0031 B0032 B0033 |
Vector using EcoRI and XbaI: | C0051 | |
Ligation | B0030+C0051 B0031+C0051 B0032+C0051 B0033+C0051 | |
Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto Amp resistant plates. |