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Team:Tsinghua/Notebook/11 September 2011
From 2011.igem.org
(Difference between revisions)
| Line 11: | Line 11: | ||
sequencing result: | sequencing result: | ||
both primer VF and VR got signal | both primer VF and VR got signal | ||
| - | pAA | + | pAA can blast with Tar gene, correct |
| + | [[pSB1C3-Ampr-Chlr-PAA]] | ||
| + | sequencing result: | ||
| + | Ampr correct | ||
| + | Chlr correct | ||
| + | PAA correct | ||
| + | [[pSB1C3-Ampr-Chlr-Tar]] | ||
| + | Ampr correct | ||
| + | Chlr correct | ||
| + | PAA correct | ||
[[pSB1C3-prorich-mcherry]] | [[pSB1C3-prorich-mcherry]] | ||
sequencing result: | sequencing result: | ||
genome!!!!! | genome!!!!! | ||
| + | [[pPD12-Tar]] | ||
| + | no sighanl. Correct cause primer are on pSB1C3 | ||
| + | [[pPD12-PAA]] | ||
| + | no sighanl. Correct cause primer are on pSB1C3 | ||
Revision as of 09:05, 11 September 2011
pSB1C3-Tar sequencing result:
tar1,4 are correct tar2 has single nucleotide addition! tar:1687bp
sequencing result: p15A 6,7 are both correct p15A 4 not good p15A:914bp
sequencing result: both primer VF and VR got signal pAA can blast with Tar gene, correct
sequencing result: Ampr correct Chlr correct PAA correct
Ampr correct Chlr correct PAA correct
sequencing result: genome!!!!!
no sighanl. Correct cause primer are on pSB1C3
no sighanl. Correct cause primer are on pSB1C3
