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Reporter: Week 3 May 30- June 4
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Revision as of 19:04, 7 June 2011
For week three, the reporter group continued creating the lac inducible test construct. When the primers, designed during week two, arrived, the reporters began assembling the linkers and cleavage sites via PCR.
Monday
Amplified Insert Assembly
| Assembly Steps | Parts Involved with Assembly Step |
|---|---|
| PCR | J33204 miniprep from 5/26/11 |
| Restriction Digest | Insert: J33204 using XbaI and PstI Vector: R0010 using SpeI and PstI |
| Ligation | J33204+R0010 digests |
| Transformation/Plating | The above ligation was transformed into Escherichia coli cells and plated on Amp resistant plates. |
