Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization

From 2011.igem.org

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<b>The system is customizable!!</b><br> <br> <br>
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<!--<span id="test">test</span>-->
 
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<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization#creating new biobricks " class="h1">Creating new parts</a><br><br>
 
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<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization#Scar-free assembly " class="h1">Scar-free assembly</a><br><br>
 
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<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization#Site-directed mutagenesis " class="h1">Site-directed mutagenesis</a><br>
 
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We know how important flexibility within molecular biology is, however setting up a standard often entails rigidity.
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<br>We therefore here provide a guide on how to customize the Plug’n’Play assembly standard.
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<b>The system is customizable!!</b><br>
 
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We know how important flexibility within molecular biology is, however setting up a standard often entails rigidity.
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<br>We therefore here provide a guide on how to customize the Plug’n’Play assembly standard.
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<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization#creating new biobricks " class="h1">Creating new parts</a><br><br>
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<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization#Scar-free assembly " class="h1">Scar-free assembly</a><br><br>
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<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization#Site-directed mutagenesis " class="h1">Site-directed mutagenesis</a><br>
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• When you want to introduce mutations
• When you want to introduce mutations
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<a name="Creating new parts"></a><h2><b>Creating new parts</b></h2>
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<a name="Seamless assembly"></a><h2><b>Seamless assembly</b></h2>
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<a name="Site-directed mutagenesis"></a><h2><b>Site-directed mutagenesis</b></h2>
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Revision as of 20:13, 10 September 2011



The system is customizable!!


We know how important flexibility within molecular biology is, however setting up a standard often entails rigidity.
We therefore here provide a guide on how to customize the Plug’n’Play assembly standard.


When do you need to customize the system?

• When a desired biological part is not yet a part of the Plug’n’Play with DNA kit
• When scar-free assembly is required (e.g. protein fusion)
• When you want to introduce mutations




Creating new parts




Seamless assembly


Site-directed mutagenesis