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Team:Cornell/Week 14
From 2011.igem.org
(Difference between revisions)
(→Tuesday, September 6) |
(→Monday, September 5) |
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Lab work done by: Youjin Cho and Claire Paduano | Lab work done by: Youjin Cho and Claire Paduano | ||
:*Poured PDMS for both of the new wafers made with Dr. Archer | :*Poured PDMS for both of the new wafers made with Dr. Archer | ||
| - | :*Hardened in | + | :*Hardened in 60°C oven overnight |
:'''Miniprep''' | :'''Miniprep''' | ||
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::- vioB+avi-tag+pZE12 (1,2,3) in DH5α | ::- vioB+avi-tag+pZE12 (1,2,3) in DH5α | ||
::- vioE+avi-tag+pZE12 (1,2,3) in DH5α | ::- vioE+avi-tag+pZE12 (1,2,3) in DH5α | ||
| - | ::- RFP+avi-tag+pZE12 (1,2,3)in | + | ::- RFP+avi-tag+pZE12 (1,2,3)in MC4100 |
| - | ::- vioA+avi-tag+pZE12 (1,2,3)in | + | ::- vioA+avi-tag+pZE12 (1,2,3)in MC4100 |
| - | ::- vioB+avi-tag+pZE12 (1,2,3)in | + | ::- vioB+avi-tag+pZE12 (1,2,3)in MC4100 |
| - | ::- vioE+avi-tag+pZE12 (1,2,3)in | + | ::- vioE+avi-tag+pZE12 (1,2,3)in MC4100 |
==Tuesday, September 6== | ==Tuesday, September 6== | ||
Revision as of 06:26, 10 September 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
September 4th - September 10th
Sunday, September 4
Monday, September 5
Lab work done by: Youjin Cho and Claire Paduano
- Poured PDMS for both of the new wafers made with Dr. Archer
- Hardened in 60°C oven overnight
- Miniprep
- - RFP+avi-tag+pZE12 (1,2,3) in DH5α
- - vioA+avi-tag+pZE12 (1,2,3) in DH5α
- - vioB+avi-tag+pZE12 (1,2,3) in DH5α
- - vioE+avi-tag+pZE12 (1,2,3) in DH5α
- - RFP+avi-tag+pZE12 (1,2,3)in MC4100
- - vioA+avi-tag+pZE12 (1,2,3)in MC4100
- - vioB+avi-tag+pZE12 (1,2,3)in MC4100
- - vioE+avi-tag+pZE12 (1,2,3)in MC4100
Tuesday, September 6
Morning lab work done by: Maneesh Gupta and Claire Paduano
- Made microfluidic chips poured yesterday
