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Revision as of 09:44, 8 September 2011
General culture conditions
Antibiotics were used at the following concentrations :
Ampicilin (Amp) : 100µg/mL
Chloramphenicol (Cm) : 20µg/mL
Spectinomycin (Spc) : 100µg/mL
Kanamycin (Kan) : 30µg/mL
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution.
The composition of the different media we have used is the following :
LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB
LB/2 : 50/50 (v/v) mix of LB and water
Choose one of our protocols to read its description
. CaCl2 chemical transformation
. Ligation
. NucleoSpin Plasmid QuickPure : Isolation of high-copy plasmid DNA from E. coli
. Plasmid or Cosmid DNA purification using HiSpeed Plasmid Midi kits
. Plasmid DNA purification using QIAprep® Spin Miniprep Kit
