Team:Yale

From 2011.igem.org

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welcome to igem yale
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    What would it take to make bacteria produce an electrical circuit? One of the most exciting uses for synthetic biology is in the design of biological systems that can replace and improve industrial processes. By achieving industrial goals using biological processes, we predict dramatic reductions in economic and environmental manufacturing costs. Our project is a first step towards biologically synthesized electronic circuits. Based on precedence of naturally redox-capable bacteria, we generated a system in E. coli that reduces metal in solution. Depending on the application, this system has the ability to form a conductive copper sulfide that can be localized with high precision. In the future our bacteria could catalyze metal deposition to form electrical circuits of any desired dimension and complexity.
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<!------------- ABSTRACT: NEEDS TO BE WRITTEN ------------->
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<a id="nav" href="https://2010.igem.org/Team:Yale/Our Project"> >> to learn more about our project: </a>
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<a id="img-2" href="https://2010.igem.org/Team:Yale/Our Project/Methods">Experimental Methods</a>
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<a id="img-3" href="https://2010.igem.org/Team:Yale/Our Project/Notebook">Lab Notebook</a>
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<title>iGEM Yale</title>
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<a id="img-4" href="https://2010.igem.org/Team:Yale/Our Project/Applications">Applications</a>
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about us
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A first year entrant to the iGEM competition, our team is comprised of students from a wide range of backgrounds connected by a common passion for synthetic biology and its applications. After much brainstorming and many wetlab sessions, our team has succeeded in developing a genomic platform for bacterial circuit construction.
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<a id="nav" href="https://2010.igem.org/Team:Yale/Our Team"> >> read more about our team </a>
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<li><a href="https://2011.igem.org/Team:Yale">Home</a></li>
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<li><a href="https://2011.igem.org/Team:Yale/Project">Our Project</a></li>
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<h3> Introduction </h3>
 
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''Please read the attached file.''
 
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<html><a href="https://static.igem.org/mediawiki/2011/9/95/Yale_iGEM_2011_Outline.ppt">Summary and Outline of 2011 Yale iGEM (PPT)</a></html>
 
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<h3>  Recent Updates </h3>
 
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''In the past month, we have:"
 
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1) Successfully expressed four different antifreeze protein constructs in BL21 and Origami strain E. coli cells (verified by observing green pellets, fluorimetry, SDS/PAGE Gel electrophoresis, and Western blotting)
 
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2) Demonstrated enhanced cold survivability at -20° C in cells expressing antifreeze protein (microbial plating assay)
 
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3) Verified sequences of all antifreeze protein constructs
 
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4) Cloned three antifreeze protein constructs into the standard iGEM BioBrick vector
 
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We have received the synthetic novel Siberian beetle antifreeze protein gene that was sponsored by IDT and have begun cloning and subsequent experiments on this construct.  We are also developing an ice recrystallization inhibition assay and finishing the cloning of several more BioBricks. Soon, we hope to begin optimizing expression of the antifreeze proteins using directed evolution technologies.
 
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<!--- The Mission, Experiments --->
 
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<h3> Active Directory </h3>
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:Yale|Home]]
 
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!align="center"|[[Team:Yale/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Yale Official Team Profile]
 
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!align="center"|[[Team:Yale/Project|Project]]
 
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!align="center"|[[Team:Yale/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:Yale/Modeling|Modeling]]
 
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!align="center"|[[Team:Yale/Notebook|Notebook]]
 
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!align="center"|[[Team:Yale/Safety|Safety]]
 
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!align="center"|[[Team:Yale/Attributions|Attributions]]
 
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|}
 

Revision as of 08:11, 8 September 2011

iGEM Yale

welcome to igem yale

What would it take to make bacteria produce an electrical circuit? One of the most exciting uses for synthetic biology is in the design of biological systems that can replace and improve industrial processes. By achieving industrial goals using biological processes, we predict dramatic reductions in economic and environmental manufacturing costs. Our project is a first step towards biologically synthesized electronic circuits. Based on precedence of naturally redox-capable bacteria, we generated a system in E. coli that reduces metal in solution. Depending on the application, this system has the ability to form a conductive copper sulfide that can be localized with high precision. In the future our bacteria could catalyze metal deposition to form electrical circuits of any desired dimension and complexity. >> to learn more about our project:

Applications
about us

A first year entrant to the iGEM competition, our team is comprised of students from a wide range of backgrounds connected by a common passion for synthetic biology and its applications. After much brainstorming and many wetlab sessions, our team has succeeded in developing a genomic platform for bacterial circuit construction. >> read more about our team

Retrieved from "http://2011.igem.org/Team:Yale"