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Team:Lyon-INSA-ENS/Realisation/Protocols
From 2011.igem.org
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<img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="/Team:Lyon-INSA-ENS/Realisation/ProtocolsFr">Version Française</a> | <img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="/Team:Lyon-INSA-ENS/Realisation/ProtocolsFr">Version Française</a> | ||
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| + | <div class="contenugrand2";> | ||
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| + | <br/> <br/> | ||
| + | <br/><br/><br/><br/><br/> | ||
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| + | <p> <font color="green" size="5"> | ||
| + | General culture conditions<br><HR> | ||
| + | </font></p> | ||
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| + | <br> <br><br> <br> | ||
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| + | <p style=" line-height : 1.5em"> Antibiotics were used at the following concentrations : <br> | ||
| + | Ampicilin (Amp) : 100µg/mL <br> | ||
| + | Chloramphenicol (Cm) : 20µg/mL <br> | ||
| + | Spectinomycin (Spc) : 100µg/mL <br> | ||
| + | Kanamycin (Kan) : 30µg/mL<br> | ||
| + | The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution. <br><br> | ||
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| + | The composition of the different media we have used is the following :<br> | ||
| + | LB :<br> | ||
| + | M63 :<br> | ||
| + | M63G :<br> | ||
| + | LB/2 :<br> | ||
| + | </p> | ||
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<div class="contenugrand2";> | <div class="contenugrand2";> | ||
Revision as of 19:32, 7 September 2011
General culture conditions
Antibiotics were used at the following concentrations :
Ampicilin (Amp) : 100µg/mL
Chloramphenicol (Cm) : 20µg/mL
Spectinomycin (Spc) : 100µg/mL
Kanamycin (Kan) : 30µg/mL
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution.
The composition of the different media we have used is the following :
LB :
M63 :
M63G :
LB/2 :
Choose one of our protocols to read its description
. CaCl2 chemical transformation
. Ligation
. NucleoSpin Plasmid QuickPure : Isolation of high-copy plasmid DNA from E. coli
. Plasmid or Cosmid DNA purification using HiSpeed Plasmid Midi kits
. Plasmid DNA purification using QIAprep® Spin Miniprep Kit
