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Revision as of 12:34, 5 September 2011 (view source) Sabineoesterle (Talk | contribs) (→Week 12: 5.9.-11.9.) ← Older edit		Revision as of 17:03, 5 September 2011 (view source) Luwidmer (Talk | contribs) Newer edit →
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-	{{:Team:ETH Zurich/Templates/Header/Overview|currPage=Journal}}	+	{{:Team:ETH Zurich/Templates/Header/Biology|currPage=Journal}}
		+	{| style="width:900px;background:#f5f5f5;text-align:justify;font-family: helvetica, arial, sans-serif; margin-top:25px; border-radius:10px;" cellspacing="15" cellpadding="5"
		+	|style="font-size:2em;" valign="top" class="biology"|Lab Journal
		+	|style="font-family: helvetica, arial, sans-serif;font-size:1.5em; border-radius:10px" align="right" class="biologyTitle"|Week [[#|1]] [[#|2]] [[#|3]] [[#|4]] [[#|5]] [[#|6]] [[#|7]] [[#|8]] [[#|9]] [[#|10]] [[#|11]] [[#|12]] [[#|13]] [[#|14]]
		+	|-
		+	|colspan="2"|'''Here you can watch how our project is coming together.'''
		+	|}
	== '''Week 1:  20.6-26.6''' ==		== '''Week 1:  20.6-26.6''' ==

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Revision as of 17:03, 5 September 2011

Lab Journal	Week 1 2 3 4 5 6 7 8 9 10 11 12 13 14
Here you can watch how our project is coming together.

Week 1: 20.6-26.6

• First meeting

• Brainstorming

Week 2: 27.6-3.7

• Brainstorming

Week 3: 4.7-10.7

• Working on the design of the system
• Design of several Operons for AlcR and several PCR primer

Week 4: 11.7-17.7

• Ordering of the LacI_M1 and codonoptimized AlcR gene
• Making of competent DH5-α cells
• Cloning of the AlcR-testsystem

Week 5: 18.7-24.7

• First test of the AlcR-system in 96-well plates

Week 6: 25.7-31.7

• Transformation of the parts from the iGEM plates

Week 7: 1.8-7.8

• Growth test of DH5-α with AlcR-system in flasks

• Ligation and Transformation of
  • λ_P and luxI
  • λ_P and lacI
  • P_lac and GFP_LVA
  • P_lux and mCherry
  • P_tet and CI -had to be redone because of point mutation in the primer (week 9)
  • P_const and luxR

• First meeting with Dr. Oliver Frey (Bio engineering laboratory, Prof. Andreas Hierlemann, D-BSSE ETHZ) to exchange ideas about microfluidic design

Week 8: 8.8-14.8

• Synthesized LacI_M1 Gen arrived

• Ligation and Transformation of
  • P_lac-GFP_LVA and Terminator
  • P_lux-mCherry and Terminator
  • P_const-luxR and Terminator
  • P_lac-GFP_LVA-Terminator and P_lux-mCherry-Terminator on one plasmid
  • P_tet and LacI_M1
  • P_tet-LacI_M1 and Terminator

• Improving of the testsystem
  • Exchange of same rare codons in AlcR by PCR
  • His-tagging of AlcR
  • GFP assam

• Transformation of λ_P

• Making of competent JM101 cells
  • Check for transformation efficiency

Week 9: 15.8-21.8

• Growth and repression test of AlcR-system with M9-medium
• Ligation and Transformation of
  • λ_P and lacI
  • λ_P and luxI
  • P_tet and CI
  • λ_P-lacI and terminator
  • λ_P-luxI and terminator

Week 10: 22.8-28.8

• Transformation and Ligation
  • P_tet-CI and Terminator
• Preparation of chemically competent DH5-α and JM101 cells

Week 11: 29.8-4.9.

• Transformation and Ligation
  • P_tet-CI and Terminator
• Diffusion test through tube system filled with agarose and cells
• Test of the improved AlcR system

Week 12: 5.9.-11.9.

• Design of a system to characterize our new biobrick LacI_M1
  • Transformation and Ligation
    • P_Tet and LacI
    • P_Lac and GFP assam

• Design of an additional construct to test the band pass filter
  • Transformation and Ligation
    • P_const and TetR_LVA

• Growth-test of E.coli in M9

• Testdigest of λP-luxI-Terminator

Week 13: 12.9.-18.9.

Week 14: 19.9.-25.9