Agenda:Transformation Test Run

(Difference between revisions)

Latest revision as of 16:36, 4 June 2011

(1) Obtained a vial of 2mL of TOP-10 electro competent E. coli cells 3-16-11 (44-0003/793762)

(2) Added 50 microliters of BIO-RAD transformation solution 
   (Control number 31000008916 recd 1-17-11 GT)

(3) Added 2 microliters of DNA from Registry plate 2  well 5b

(4) Incubate on ice 5 minutes.

(5) Heat shocked 40 degrees C for 50 seconds

(6) Incubated 4 degrees C for 50 seconds

(7) Added 1mL LB 5-17-11 D.G.

(8) Plate 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "winterkist")

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

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   (8) Plate 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "winterkist")
+{| style="color:#008080;background-color:#7CFC00;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2|Home]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Team|Team]]
+!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=SouthBend-Mishawaka-HS-2 Official Team Profile]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Project|Project]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Parts|Parts Submitted to the Registry]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Modeling|Modeling]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Notebook|Notebook]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Safety|Safety]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Attributions|Attributions]]
+|}