Team:Osaka/week6

From 2011.igem.org

(Difference between revisions)
Line 15: Line 15:
!Name!!IPTG(0.1M)!!incubation!!OD600!!A474
!Name!!IPTG(0.1M)!!incubation!!OD600!!A474
|-
|-
-
|1||-||2h||0.8583||0.1567
+
|1|| -||2h||0.8583||0.1567
|-
|-
-
|2||400μl||2h||1.1523||0.1567
+
|2||400μl||2h||1.1523||0.1447
|-
|-
-
|3||400μl||1h||0.5583||0.1567
+
|3||400μl||1h||0.5583||0.1126
|-
|-
-
|4||40μl||2h||1.0297||0.1567
+
|4||40μl||2h||1.0297||0.1878
|-
|-
-
|5||40μl||1h||0.6576||0.1567
+
|5||40μl||1h||0.6576||0.1736
|}
|}

Revision as of 12:34, 5 September 2011


Contents

September 4(Sun)

  1. Restriction digests:3-10C,1-2M
  2. Gel extraction of digests
  3. Ligation
    • 03:1-9N+3-6H again
  4. Transformation of ligation products
  5. Photospectrometry measuament

September 5(Mon)

Table 1
NameIPTG(0.1M)incubationOD600A474
1 -2h0.85830.1567
2400μl2h1.15230.1447
3400μl1h0.55830.1126
440μl2h1.02970.1878
540μl1h0.65760.1736


September 6(Tue)

September 7(Wed)

September 8(Thu)

September 9(Fri)

September 10(Sat)

Back to Notebook

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