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Team:Osaka/week6
From 2011.igem.org
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| - | ! | + | !Name!!IPTG(0.1M)!!incubation!!OD600!!A474 |
|- | |- | ||
|1-9N||<bbpart>BBa_J22106</bbpart>||A|| rec A (SOS) Promoter | |1-9N||<bbpart>BBa_J22106</bbpart>||A|| rec A (SOS) Promoter | ||
Revision as of 12:30, 5 September 2011
Contents |
September 4(Sun)
- Restriction digests:3-10C,1-2M
- Gel extraction of digests
- Ligation
- 03:1-9N+3-6H again
- Transformation of ligation products
- Photospectrometry measuament
September 5(Mon)
| Name | IPTG(0.1M) | incubation | OD600 | A474 |
|---|---|---|---|---|
| 1-9N | <bbpart>BBa_J22106</bbpart> | A | rec A (SOS) Promoter | |
| 1-2M | <bbpart>BBa_K118022</bbpart> | A | RBS | |
| 1-14K | <bbpart>BBa_B0040</bbpart> | A | green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP | |
| 1-12M | <bbpart>BBa_E0240</bbpart> | A | GFP generator | |
| 1-1D | <bbpart>BBa_R0010</bbpart> | A | promoter(lacI regulatd) |
