Revision as of 09:53, 5 September 2011

Data

Plasmid backbone: pSB3K1 from ETHZ 2007 [http://partsregistry.org/wiki/index.php?title=Part:pSB3K1 "pSB3K1"] (taken from the delivery plate)
Pconst: J23116 from Berkeley 2006 [http://partsregistry.org/Part:BBa_J23116 "j23116"] (sequence copied into our primers)
RBS and spacer: (sequence copied into our primers)
TetR: "TetR sequence" (623 bp) The sequence lacks a stop codon, we added TAA with our primers

Sequenced data compared to the sequence of Pconst,RBS+spacer and TetR gene:"pSb3K1_TetR_seq" All these parts are correct.

Parts assembled

Plasmid backbone containing Pconst and TetR: see precedent section
Terminator: B0014 from the Registry [http://partsregistry.org/Part:BBa_B0014 "B0014"] (sequence copied into our primers)
Ptet: R0040 from Registry [http://partsregistry.org/Part:BBa_R0040 "R0040"] (sequence copied into our primers)
LacI: amplified from Repressilator plasmid

Sequencing results:

@@ Line 52: / Line 52: @@
 Parts assembled
 * Plasmid backbone containing Pconst and TetR: see precedent section
-* Terminator: B0014 from the Registry [http://partsregistry.org/Part:BBa_B0014] (sequence copied into our primers)
+* Terminator: B0014 from the Registry [http://partsregistry.org/Part:BBa_B0014 "B0014"] (sequence copied into our primers)
-* Ptet: R004 from Registry [http://partsregistry.org/Part:BBa_R0040] (sequence copied into our primers)
+* Ptet: R0040 from Registry [http://partsregistry.org/Part:BBa_R0040 "R0040"] (sequence copied into our primers)
 * LacI: amplified from Repressilator plasmid