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Team:EPF-Lausanne/Our Project/Data
From 2011.igem.org
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[[File:EPFL_Tetr_plasmid.jpg|300px]] | [[File:EPFL_Tetr_plasmid.jpg|300px]] | ||
| - | Parts assembled: | + | ====Parts assembled:==== |
* Plasmid backbone: pSB3K1 from ETHZ 2007 [http://partsregistry.org/wiki/index.php?title=Part:pSB3K1] (taken from the delivery plate) | * Plasmid backbone: pSB3K1 from ETHZ 2007 [http://partsregistry.org/wiki/index.php?title=Part:pSB3K1] (taken from the delivery plate) | ||
* Pconst: J23116 from Berkeley 2006 [http://partsregistry.org/Part:BBa_J23116] (sequence copied into our primers) | * Pconst: J23116 from Berkeley 2006 [http://partsregistry.org/Part:BBa_J23116] (sequence copied into our primers) | ||
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| - | Sequencing results: | + | ====Sequencing results:==== |
Sequenced data compared to the sequence of Pconst,RBS+spacer and TetR gene: | Sequenced data compared to the sequence of Pconst,RBS+spacer and TetR gene: | ||
| + | [[File:EPFL_pSb3K1_TetR_seq.txt]] | ||
All these parts are correct. | All these parts are correct. | ||
Revision as of 09:39, 5 September 2011
Data
Contents |
TetR Mutants
- V36F
- P39K
- Y42F
- P39Q-Y42M
T7 Promoter Plasmids
- pSB3K1-T7-const-RFP
- pSB3K1-T7-lac-RFP
- pSB3K1-T7-lac2-RFP
- pSB3K1-T7-const-Lysis
- pSB3K1-T7-lac-Lysis
- pSB3C5-T7-const-RFP
- pSB3C5-T7-lac-RFP
- pSB3C5-T7-lac2-RFP
- pSB3C5-T7-lac-Lysis
- pSB3C5-T7-lac2-Lysis
T7 Promoter Variants
Reporter Plasmids
J61002 Ptet-RFP
TetR plasmids
pSB3K1 Pconst-TetR
Parts assembled:
- Plasmid backbone: pSB3K1 from ETHZ 2007 [http://partsregistry.org/wiki/index.php?title=Part:pSB3K1] (taken from the delivery plate)
- Pconst: J23116 from Berkeley 2006 [http://partsregistry.org/Part:BBa_J23116] (sequence copied into our primers)
- RBS and spacer: (sequence copied into our primers)
- TetR: from Alina?
Sequencing results:
Sequenced data compared to the sequence of Pconst,RBS+spacer and TetR gene: File:EPFL pSb3K1 TetR seq.txt All these parts are correct.
pSB3K1 Pconst-TetR Ptet-LacI
Parts assembled
- Plasmid backbone containing Pconst and TetR: see precedent section
- Terminator: B0014 from the Registry [http://partsregistry.org/Part:BBa_B0014] (sequence copied into our primers)
- Ptet: R004 from Registry [http://partsregistry.org/Part:BBa_R0040] (sequence copied into our primers)
- LacI: amplified from Repressilator plasmid
Sequencing results:
