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Team:Paris Liliane Bettencourt/Notebook/2011/09/04/
From 2011.igem.org
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===Biobrick the YFP:tetR fusion protein === | ===Biobrick the YFP:tetR fusion protein === | ||
| - | Let's start again with | + | Let's start again with good primers to biobrick the protein. |
====PCR==== | ====PCR==== | ||
Revision as of 13:59, 4 September 2011
Contents |
Kevin
Biobrick the YFP:tetR fusion protein
Let's start again with good primers to biobrick the protein.
PCR
3 Temperatures of primer annealing: 85 - 58 - 48 °C
Primers : pFX234 FW - pFX234 Reverse CP
Following steps
PCR Purification, digestion in ES, PCR Purification again, Ligation
To be continued
Transformation, sequencing and glycerol.
