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Team:UST-Beijing/Notebook
From 2011.igem.org
(Difference between revisions)
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<p><h1>Project 2.</h1></p> | <p><h1>Project 2.</h1></p> | ||
<p><h2>Step 1：The construction of pSB1AC3/PR</h2></p> | <p><h2>Step 1：The construction of pSB1AC3/PR</h2></p> | ||
| - | <table align="left" border="0" cellpadding="0" cellspacing=" | + | <table align="left" border="0" cellpadding="0" cellspacing="0" > |
<tr> | <tr> | ||
<td> <img src="https://static.igem.org/mediawiki/2011/7/71/1.1PR%E7%89%87%E6%AE%B5.jpg" width="250" height="150"/> </td> | <td> <img src="https://static.igem.org/mediawiki/2011/7/71/1.1PR%E7%89%87%E6%AE%B5.jpg" width="250" height="150"/> </td> | ||
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| - | < | + | <p> </p> |
| - | < | + | <p> </p> |
| - | + | <p> </p> | |
| - | + | <p> </p> | |
| - | + | <p>PR + pSB1AC3 = pSB1AC3/PR (new part)</p> | |
| - | + | <p>Cut PR w/EcoRI & SpeI</p> | |
| - | + | <p>Cut pSB1AC3 w/EcoRI & SpeI</p> | |
| - | PR + pSB1AC3 = pSB1AC3/PR (new part) | + | <p>Combine the insert and vector with T4 ligase</p> |
| - | Cut PR w/EcoRI & SpeI | + | |
| - | Cut pSB1AC3 w/EcoRI & SpeI | + | |
| - | Combine the insert and vector with T4 ligase | + | |
| - | </ | + | |
<p><h2>Step2: Gel electrophoresis</h2></p><br /> | <p><h2>Step2: Gel electrophoresis</h2></p><br /> | ||
<table align="left" border="0" cellpadding="5" cellspacing="5" > | <table align="left" border="0" cellpadding="5" cellspacing="5" > | ||
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</table> | </table> | ||
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| + | <br /><br /> | ||
<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
| - | |||
| - | |||
<p><h2>Step 3: DNA sequencing<h2></p> | <p><h2>Step 3: DNA sequencing<h2></p> | ||
<img src="https://static.igem.org/mediawiki/2011/3/39/3%E6%B5%8B%E5%BA%8F%E7%BB%93%E6%9E%9C.PNG" width="640"/><br /> | <img src="https://static.igem.org/mediawiki/2011/3/39/3%E6%B5%8B%E5%BA%8F%E7%BB%93%E6%9E%9C.PNG" width="640"/><br /> | ||
Revision as of 10:52, 4 September 2011
This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.
Project 2.
Step 1：The construction of pSB1AC3/PR
 |
 |
 |
PR + pSB1AC3 = pSB1AC3/PR (new part)
Cut PR w/EcoRI & SpeI
Cut pSB1AC3 w/EcoRI & SpeI
Combine the insert and vector with T4 ligase
Step2: Gel electrophoresis
 |
1: wide range maker
2、3、4、5：the constructed plasmid cutted with SpeI and EcoRI restriction enzymes |
Step 3: DNA sequencing
The result of DNA sequencing demonstrates that there is no mutation in the new part by comparing to the original sequence.
The result of DNA sequencing demonstrates that there is no mutation in the new part by comparing to the original sequence.
Notebook
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
