Team:Paris Liliane Bettencourt/Notebook/2011/09/04

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(Difference between revisions)

Revision as of 22:54, 3 September 2011

Danyel

Overview

The constructs involved in the tRNA project so far:

General information
- tRNA = approx. 100 bp
- T7 amber = approx. 2.7 kb
- GFP amber = approx. 720 bp

tRNA amber suppressor
- tRNA

     S38: in pSB1C3

- tRNA_TT

     S58: in pSB1C3

- Pveg_spoVG_tRNA

     S38 in S24 --- [in progress]

- Pveg_spoVG_tRNA_TT

     S81 :  this contruct in pSB1AK3 (ampR)
     S92 :  this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.

- pHyperspank_tRNA

     S38 in S82 --- [in progress]

- pHyperspank_tRNA_TT

     S58 in S82 --- [in progress]

- pT7_RBS_GFP_TT :

     S79 :  this construct in ampR plasmid (pSB1AK3?).

- - functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)

Proteins with amber mutations
T7 amber_TT
- S80 : in pSB1AK3

@@ Line 10: / Line 10: @@
 **tRNA
        S38: in pSB1C3
-**tRNA_TT | S58: in pSB1C3
+**tRNA_TT
-**Pveg_spoVG_tRNA | S38 in S24 --- [in progress]
+      S58: in pSB1C3
+**Pveg_spoVG_tRNA
+      S38 in S24 --- [in progress]
 **Pveg_spoVG_tRNA_TT
-***S81 :  this contruct in pSB1AK3 (ampR)
+      S81 :  this contruct in pSB1AK3 (ampR)
-***S92 :  this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.
+      S92 :  this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.
 **pHyperspank_tRNA
-*** S38 in S82 --- [in progress]
+      S38 in S82 --- [in progress]
 **pHyperspank_tRNA_TT
-*** S58 in S82 --- [in progress]
+      S58 in S82 --- [in progress]
 **pT7_RBS_GFP_TT  :
-***S79 :  this construct in ampR plasmid (pSB1AK3?).
+      S79 :  this construct in ampR plasmid (pSB1AK3?).
 ***functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)