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Team:Paris Liliane Bettencourt/Notebook/2011/09/04
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===Overview=== | ===Overview=== | ||
The constructs involved in the tRNA project so far: | The constructs involved in the tRNA project so far: | ||
| + | |||
| + | '''tRNA amber suppressor''' | ||
| + | General information: | ||
| + | tRNA = approx. 100 bp | ||
*tRNA | *tRNA | ||
** S38: in pSB1C3 | ** S38: in pSB1C3 | ||
| Line 18: | Line 22: | ||
**S79 : this construct in ampR plasmid (pSB1AK3?). | **S79 : this construct in ampR plasmid (pSB1AK3?). | ||
**functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11) | **functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11) | ||
| + | |||
| + | '''Proteins with amber mutations''' | ||
| + | * T7 amber_TT | ||
| + | ** S80 : in pSB1AK3 | ||
Revision as of 22:47, 3 September 2011
Danyel
Overview
The constructs involved in the tRNA project so far:
tRNA amber suppressor General information: tRNA = approx. 100 bp
- tRNA
- S38: in pSB1C3
- tRNA_TT
- S58: in pSB1C3
- Pveg_spoVG_tRNA
- S38 in S24 --- [in progress]
- Pveg_spoVG_tRNA_TT
- S81 : this contruct in pSB1AK3 (ampR)
- S92 : this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.
- pHyperspank_tRNA
- S38 in S82 --- [in progress]
- pHyperspank_tRNA_TT
- S58 in S82 --- [in progress]
- pT7_RBS_GFP_TT :
- S79 : this construct in ampR plasmid (pSB1AK3?).
- functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)
Proteins with amber mutations
- T7 amber_TT
- S80 : in pSB1AK3
