Team:Paris Liliane Bettencourt/Notebook/2011/09/04

(Difference between revisions)

Revision as of 22:43, 3 September 2011

The constructs involved in the tRNA project so far:

tRNA
- S38: in pSB1C3
tRNA_TT
- S58: in pSB1C3
Pveg_spoVG_tRNA
- S38 in S24 --- [in progress]
Pveg_spoVG_tRNA_TT
- S81 : this contruct in pSB1AK3 (ampR)
- S92 : this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.
pHyperspank_tRNA
- S38 in S82 --- [in progress]
pHyperspank_tRNA_TT
- S58 in S82 --- [in progress]
pT7_RBS_GFP_TT :
- S79 : this construct in ampR plasmid (pSB1AK3?).
- functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)

@@ Line 2: / Line 2: @@
 ===Overview===
 The constructs involved in the tRNA project so far:
-*tRNA : S38
+*tRNA
-** in pSB1C3
+** S38: in pSB1C3
-*tRNA_TT : S58
+*tRNA_TT
-** in pSB1C3
+** S58: in pSB1C3
-*Pveg_spoVG_tRNA : S38 in S24 --- [in progress]
+*Pveg_spoVG_tRNA
+** S38 in S24 --- [in progress]
 *Pveg_spoVG_tRNA_TT
 **S81 :  this contruct in pSB1AK3 (ampR)
 **S92 :  this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.
-*pHyperspank_tRNA  :  S38 in S82 --- [in progress]
+*pHyperspank_tRNA
-*pHyperspank_tRNA_TT  :  S58 in S82 --- [in progress]
+** S38 in S82 --- [in progress]
+*pHyperspank_tRNA_TT
+** S58 in S82 --- [in progress]
 *pT7_RBS_GFP_TT  :
 **S79 :  this construct in ampR plasmid (pSB1AK3?).
 **functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)